Molecular Biology Reports

, Volume 40, Issue 2, pp 1397–1405

Identification of genes differentially expressed in the roots of rubber tree (Hevea brasiliensis Muell. Arg.) in response to phosphorus deficiency

  • Peng He
  • Huaide Qin
  • Min Wu
  • Bingsun Wu
  • Jiashao Wei
  • Dapeng Wang
Article
  • 459 Downloads

Abstract

Phosphorus (P) is an essential macronutrient for plant growth and development. P deficiency could affect rubber tree productivity seriously, and understanding the mechanism responses of the rubber tree under the P deficiency will be helpful to improving rubber tree productivity. The molecular mechanism by which the rubber trees respond to a P-deficiency is a complex network involving many processes. To identify the genes differentially expressed in that response, we constructed subtractive suppression hybridization libraries for roots of plants growing under deficient or sufficient conditions. We identified 94 up-regulated genes from the forward library and 45 down-regulated from the reverse library. These differentially expressed genes were categorized into eight groups representing functions in metabolism, transcription, signal transduction, protein synthesis, transport, stress responses, photosynthesis, and development. We also performed quantitative real-time PCR to investigate the expression profiles of eight randomly selected clones. Our results provide useful information for further study of the molecular mechanism for adaptations to a P-deficiency in this species. Further characterization and functional analysis of these differentially expressed genes will help us improve its phosphorus utilization and overall productivity.

Keywords

Rubber tree Phosphorus deficiency Subtractive suppression hybridization Differentially expressed gene 

References

  1. 1.
    Marschner H (1995) Mineral nutrition of higher plants, Ed 2. Academic Press/Harcourt Brace, LondonGoogle Scholar
  2. 2.
    Raghothama KG (1999) Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol 50:665–693PubMedCrossRefGoogle Scholar
  3. 3.
    Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol 157:423–447CrossRefGoogle Scholar
  4. 4.
    Poirier Y, Bucher M (2002) Phosphate transport and homeostasis in Arabidopsis. In: Somerville CR, Meyerowitz EM (eds) The Arabidopsis book. American Society of Plant Biologists, Rockville MDGoogle Scholar
  5. 5.
    Rouached H, Arpat AB, Poirier Y (2010) Regulation of phosphate starvation responses in plants: signaling players and cross-talks. Mol Plant 3(2):288–299PubMedCrossRefGoogle Scholar
  6. 6.
    Misson J, Raghothama KG, Jain A, Jouhet J, Block MA, Bligny R et al (2005) A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proc Natl Acad Sci USA 102(33):11934–11939PubMedCrossRefGoogle Scholar
  7. 7.
    Hammond JP, Broadley MR, Bowen HC, Spracklen WP, Hayden RM, White PJ (2011) Gene expression changes in phosphorus deficient potato (Solanum tuberosum L.) leaves and the potential for diagnostic gene expression markers. PLoS ONE 6(9):e24606PubMedCrossRefGoogle Scholar
  8. 8.
    Li L, Liu C, Lian X (2010) Gene expression profiles in rice roots under low phosphorus stress. Plant Mol Biol 72(4–5):423–432PubMedCrossRefGoogle Scholar
  9. 9.
    Ai P, Sun S, Zhao J, Fan X, Xin W, Guo Q, Yu L, Shen Q, Wu P, Miller AJ et al (2009) Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation. Plant J 57:798–809PubMedCrossRefGoogle Scholar
  10. 10.
    Jia H, Ren H, Gu M, Zhao J, Sun S, Chen J, Wu P, Xu G (2011) Phosphate transporter gene, OsPht1;8, is involved in phosphate homeostasis in rice. Plant Physiol 156:1164–1175PubMedCrossRefGoogle Scholar
  11. 11.
    Nagarajan VK, Jain A, Poling MD, Lewis AJ, Raghothama KG, Smith AP (2011) Arabidopsis Pht1;5 mobilizes phosphate between source and sink organs and influences the interaction between phosphate homeostasis and ethylene signaling. Plant Physiol 156(3):1149–1163PubMedCrossRefGoogle Scholar
  12. 12.
    Veljanovski V, Vanderbeld B, Knowles VL, Snedden WA, Plaxton WC (2006) Biochemical and molecular characterization of AtPAP26, a vacuolar purple acid phosphatase up-regulated in phosphate-deprived Arabidopsis suspension cells and seedlings. Plant Physiol 142(3):1282–1293PubMedCrossRefGoogle Scholar
  13. 13.
    Liang C, Tian J, Lam HM, Lim BL, Yan X, Liao H (2010) Biochemical and molecular characterization of PvPAP3, a novel purple acid phosphatase isolated from common bean enhancing extracellular ATP utilization. Plant Physiol 152(2):854–865PubMedCrossRefGoogle Scholar
  14. 14.
    Hur YJ, Jin BR, Nam J, Chung YS, Lee JH, Choi HK, Yun DJ, Yi G, Kim YH, Kim DH (2010) Molecular characterization of OsPAP2: transgenic expression of a purple acid phosphatase up-regulated in phosphate-deprived rice suspension cells. Biotechnol Lett 32(1):163–170PubMedCrossRefGoogle Scholar
  15. 15.
    Rubio V, Linhares F, Solano R, Martin AC, Iglesias J, Leyva A, Paz-Ares J (2001) A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes Dev 15:2122–2133PubMedCrossRefGoogle Scholar
  16. 16.
    Gonzalez E, Solano R, Rubio V, Leyva A, Paz-Ares J (2005) PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 is a plant-specific SEC12-related protein that enables the endoplasmic reticulum exit of a high affinity phosphate transporter in Arabidopsis. Plant Cell 17:3500–3512PubMedCrossRefGoogle Scholar
  17. 17.
    Yi K, Wu Z, Zhou J, Du L, Guo L, Wu Y, Wu P (2005) OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Plant Physiol 138(4):2087–2096PubMedCrossRefGoogle Scholar
  18. 18.
    Wang C, Ying S, Huang H, Li K, Wu P, Shou H (2009) Involvement of OsSPX1 in phosphate homeostasis in rice. Plant J 57:895–904PubMedCrossRefGoogle Scholar
  19. 19.
    Chen J, Liu Y, Ni J, Wang Y, Bai Y, Shi J, Gan J, Wu Z, Wu P (2011) OsPHF1 regulates the plasma membrane localization of low- and high-affinity inorganic phosphate transporters and determines inorganic phosphate uptake and translocation in rice. Plant Physiol 157(1):269–278PubMedCrossRefGoogle Scholar
  20. 20.
    Zhou J, Jiao F, Wu Z, Li Y, Wang X, He X, Zhong W, Wu P (2008) OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants. Plant Physiol 146(4):1673–1686PubMedCrossRefGoogle Scholar
  21. 21.
    Mewes HW, Frishman D, Guldener U, Mannhaupt G, Mayer K et al (2002) MIPS: a database for genomes and protein sequences. Nucleic Acids Res 30:31–34PubMedCrossRefGoogle Scholar
  22. 22.
    Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−△△ct method. Methods 25:402–408PubMedCrossRefGoogle Scholar
  23. 23.
    Morcuende R, Bari R, Gibon Y, Zheng W, Pant BD, Bläsing O, Usadel B et al (2007) Genome-wide reprogramming of metabolism and regulatory networks of Arabidopsis in response to phosphorus. Plant Cell Environ 30(1):85–112PubMedCrossRefGoogle Scholar
  24. 24.
    Guo W, Zhang L, Zhao J, Liao H, Zhuang C, Yan XL (2008) Identification of temporally and spatially phosphate-starvation responsive genes in Glycine max. Plant Sci 175:574–584CrossRefGoogle Scholar
  25. 25.
    Kitomi Y, Ito H, Hobo T et al (2011) The auxin responsive AP2/ERF transcription factor CROWN ROOTLESS5 is involved in crown root initiation in rice through the induction of OsRR1, a type-A response regulator of cytokinin signaling. Plant J 67:472–484PubMedCrossRefGoogle Scholar
  26. 26.
    Kim MJ, Ruzicka D, Shin R, Schachtman DP (2012) The Arabidopsis AP2/ERF transcription factor RAP2.11 modulates plant response to low-potassium conditions. Mol Plant. doi:10.1093/mp/sss003 Google Scholar
  27. 27.
    Uematsu K, Suzuki N, Iwamae T, Inui M, Yukawa H (2012) Increased fructose 1,6-bisphosphate aldolase in plastids enhances growth and photosynthesis of tobacco plants. J Exp Bot 63(8):3001–3009PubMedCrossRefGoogle Scholar
  28. 28.
    Fan W, Zhang Z, Zhang Y (2009) Cloning and molecular characterization of fructose-1,6-bisphosphate aldolase gene regulated by high-salinity and drought in Sesuvium portulacastrum. Plant Cell Rep 28:975–984PubMedCrossRefGoogle Scholar
  29. 29.
    Hur YJ, Yi YB, Lee JH et al (2012) Molecular cloning and characterization of OsUPS, a U-box containing E3 ligase gene that respond to phosphate starvation in rice (Oryza sativa). Mol Biol Rep 39(5):5883–5888PubMedCrossRefGoogle Scholar
  30. 30.
    Li H, Pinot F, Sauveplane V, Werck-Reichhart D, Diehl P, Schreiber L et al (2010) Cytochrome P450 family member CYP704B2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice. Plant Cell 22(1):173–190PubMedCrossRefGoogle Scholar
  31. 31.
    Harvey PJ, Campanella BF, Castro PML, Harms H, Lichtfouse E et al (2002) Phytoremediation of polyaromatic hydrocarbons, anilines and phenols. Environ Sci Pollut Res Int 9:29–47PubMedCrossRefGoogle Scholar
  32. 32.
    Kim KY, Park SW, Chung YS et al (2004) Molecular cloning of low-temperature-inducible ribosomal proteins from soybean. J Exp Bot 55(399):1153–1155PubMedCrossRefGoogle Scholar
  33. 33.
    Fu ZY, Zhang ZB, Hu XJ, Shao HB, Ping X (2009) Cloning, identification, expression analysis and phylogenetic relevance of two NADP-dependent malic enzyme genes from hexaploid wheat. C R Biol 332(7):591–602PubMedCrossRefGoogle Scholar
  34. 34.
    Detarsio E, Maurino VG, Alvarez CE, Müller GL, Andreo CS, Drincovich MF (2008) Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots. Plant Mol Biol 68(4–5):355–367PubMedCrossRefGoogle Scholar
  35. 35.
    Liu S, Cheng Y, Zhang X, Guan Q, Nishiuchi S, Hase K, Takano T (2007) Expression of an NADP-malic enzyme gene in rice (Oryza sativa L) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance. Plant Mol Biol 64(1–2):49–58PubMedCrossRefGoogle Scholar
  36. 36.
    Müller GL, Drincovich MF, Andreo CS, Lara MV (2008) Nicotiana tabacum NADP-malic enzyme: cloning, characterization and analysis of biological role. Plant Cell Physiol 49(3):469–480PubMedCrossRefGoogle Scholar
  37. 37.
    Davies PJ (1995) The plant hormones: their nature, occurrence and functions. In: Davies PJ (ed) Plant hormones: physiology, biochemistry and molecular biology. Kluwer Academic Publishers, DordrechtGoogle Scholar
  38. 38.
    Qi Y, Wang S, Shen C, Zhang S, Chen Y, Xu Y, Liu Y, Wu Y, Jiang D (2012) OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa). New Phytol 193(1):109–120PubMedCrossRefGoogle Scholar
  39. 39.
    Werner T, Nehnevajova E, Köllmer I, Novák O, Strnad M et al (2010) Root-specific reduction of cytokinin causes enhanced root growth, drought tolerance, and leaf mineral enrichment in Arabidopsis and tobacco. Plant Cell 22:3905–3920PubMedCrossRefGoogle Scholar
  40. 40.
    Schilmiller AL, Koo AJ, Howe GA (2007) Functional diversification of acyl-coenzyme A oxidases in jasmonic acid biosynthesis and action. Plant Physiol 143(2):812–824PubMedCrossRefGoogle Scholar
  41. 41.
    Kim MC, Kim TH, Park JH et al (2007) Expression of rice acyl-CoA oxidase isoenzymes in response to wounding. J Plant Physiol 164(5):665–668PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Peng He
    • 1
  • Huaide Qin
    • 1
  • Min Wu
    • 1
  • Bingsun Wu
    • 1
  • Jiashao Wei
    • 1
  • Dapeng Wang
    • 1
  1. 1.Ministry of Agriculture Key Laboratory for Rubber BiologyRubber Research Institute, Chinese Academy of Tropical Agricultural SciencesDanzhouChina

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