Abstract
Chicken prolactin (PRL) is a physiological candidate gene for egg production. Variations of T8052C and G8113C in exon 5 of PRL gene may associate with chicken egg production. The objective of the study was to investigate the association of these two single nucleotide polymorphisms in PRL gene with egg production of Recessive White chickens and Qingyuan Partridge chickens. Genotyping was performed by polymerase chain reaction-ligase detection reaction (PCR-LDR) method. The T8052C and G8113C of PRL were significantly associated with age at first egg (AFE) and total egg number at 300 days of age (EN 300). A significant association was also found between T8052C–G8113C haplotypes and AFE as well as EN300, the H2H3 was the most advantageous diplotype for egg production. We putatively drew the conclusion that these two SNPs in PRL gene as well as their haplotypes could be used as the potential molecular markers for egg production traits in chicken.
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References
Sharp PJ, Dawson A, Lea RW (1998) Control of luteinizing hormone and prolactin secretion in birds. Comp Biochem Physiol C 119:275–282
Sharp PJ (2004) Genes for persistency of egg laying: white Leghorns and broodiness. Roslin Institute Edinburgh Annual Report, pp 38–42
Romanov MN, Talbot RT, Wilson PW, Sharp PJ (1999) Inheritance of broodiness in the domestic fowl. Br Poult Sci 40(Suppl):20–21
Romanov MN, Talbot RT, Wilson PW, Sharp PJ (2002) Genetic control of incubation behavior in the domestic hen. Poult Sci 81:928–931
Cui JX, Du HL, Liang Y, Deng XM, Li N, Zhang XQ (2006) Association of polymorphisms in the promoter region of chicken prolactin with egg production. Poult Sci 85:26–31
Bhattacharya TK, Chatterjee RN, Sharma RP, Niranjan M, Rajkumar U (2011) Associations between novel polymorphisms at the 5′-UTR region of the prolactin gene and egg production and quality in chickens. Theriogenology 75(4):655–661
Bhattacharya TK, Chatterjee RN, Sharma RP, Rajkumar U, Niranjan M, Reddy BL (2011) Association of polymorphism in the prolactin promoter and egg quality traits in laying hens. Br Poult Sci 52(5):551–557
Bhattacharya TK, Chatterjee RN, Sharma RP, Niranjan M, Rajkumar U, Reddy BL (2011) Identification of haplotypes in promoter of prolactin gene and their effect on egg production and quality traits in layer chicken. Anim Biotechnol 22(2):71–86
Ishida H, Shimada K, Sato K, Seo H, Murata Y, Matsui N, Zadworny D (1991) Developmental expression of the prolactin gene in the chicken. Gen Comp Endocrinol 83:463–467
Shimada K, Ishida H, Sato K, Seo H, Matsui N (1991) Expression of PRL gene in incubation hens. J Reprod Fertil 91:147–154
Wong EA, Ferrin NH, Silaby JL, El Halawani ME (1991) Cloning of a turkey PRL cDNA: expression of PRL hormone through the reproductive cycle of the domestic turkey (Meleagris gallopavo). Gen Comp Endocrinol 83:18–26
Talbot RT, Sharp PJ (1994) A radioimmunoassay for recombinant-derived chicken prolactin suitable for the measurement of prolactin in other avian species. Gen Comp Endocrinol 96:361–369
Sharp PJ, MacNamee MC, Talbot RT, Sterling RJ, Hall TR (1984) Aspects of the neuroendocrine control of ovulation and broodiness in the domestic hen. J Exp Zool 232:475–483
Nestor KE (1980) Genetics of growth and reproduction in the turkey. 7. Relationship of total egg production, intensity of lay, broodiness, and body weight. Poult Sci 59:1385–1394
Xu GF, Chen KW (2003) Photograph album of China indigenous poultry breeds. China Agricultural Press, Beijing, pp 40–41
Barany F, Gelfand DH (1991) Cloning, overexpression and nucleotide sequence of a thermostable DNA ligase-encoding gene. Gene 109:1–11
Erehehuara (2003) Studies on the SNP of the chicken’s prolactin gene. Ph.D. Thesis, Huazhong Agricultural University
Stephens M, Smith NJ, Donnelly P (2001) A new statistical method for haplotype reconstruction from population data. Am J Hum Genet 68:978–989
Khanna M, Park P, Zirvi M, Cao WG, Picon A, Day J, Paty P, Barany F (1999) Multiplex PCR/LDR for detection of K-ras mutations in primary colon tumors. Oncogene 18:27–38
Rondini S, Pingle MR, Das S, Tesh R, Rundell MS, Hom J, Stramer S, Turner K, Rossmann SN, Lanciotti R, Spier EG, Muñoz J, Larone D, Spitzer E, Barany F, Golightly LM (2008) Development of a multiplex PCR/LDR assay for detection of West Nile Virus. J Clin Microbiol 46(7):2269–2279
Elkins PA, Christinger HW, Sandowski Y, Sakal E, Gertler A, de Vos AM, Kossiakoff AA (2000) Ternary complex between placental lactogen and the extracellular domain of the prolactin receptor. Nat Struct Biol 7:808–815
Cardon LR, Abecasis GR (2003) Using haplotype blocks to map human complex trait loci. Trends Genet 19:135–140
Lin DY (2004) Haplotype-based association analysis in cohort studies of unrelated individuals. Genet Epidemiol 26:255–264
Acknowledgments
This research was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System and National High Technology Research and Development Program 863 (2011AA100301, 2011AA100305).
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Li, HF., Shu, JT., Du, YF. et al. Analysis of the genetic effects of prolactin gene polymorphisms on chicken egg production. Mol Biol Rep 40, 289–294 (2013). https://doi.org/10.1007/s11033-012-2060-7
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DOI: https://doi.org/10.1007/s11033-012-2060-7