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Expression profile analysis of genes involved in horizontal gravitropism bending growth in the creeping shoots of ground-cover chrysanthemum by suppression subtractive hybridization

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Abstract

The molecular mechanisms underlying gravitropic bending of shoots are poorly understood and how genes related with this growing progress is still unclear. To identify genes related to asymmetric growth in the creeping shoots of chrysanthemum, suppression subtractive hybridization was used to visualize differential gene expression in the upper and lower halves of creeping shoots of ground-cover chrysanthemum under gravistimulation. Sequencing of 43 selected clones produced 41 unigenes (40 singletons and 1 unigenes), which were classifiable into 9 functional categories. A notable frequency of genes involve in cell wall biosynthesis up-regulated during gravistimulation in the upper side or lower side were found, such as beta tubulin (TUB), subtilisin-like protease (SBT), Glutathione S-transferase (GST), and expensing-like protein (EXP), lipid transfer proteins (LTPs), glycine-rich protein (GRP) and membrane proteins. Our findings also highlighted the function of some metal transporter during asymmetric growth, including the boron transporter (BT) and ZIP transporter (ZT), which were thought primarily for maintaining the integrity of cell walls and played important roles in cellulose biosynthesis. CmTUB (beta tubulin) was cloned, and the expression profile and phylogeny was examined, because the cytoskeleton of plant cells involved in the plant gravitropic bending growth is well known.

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Acknowledgments

This work was supported by a grant from the Ph.D. Programs Foundation of Ministry of Education of China (No. 20100097110002); the Program for New Century Excellent Talents in University of Chinese Ministry of Education (Grant No. NCET-10-0492); the Fundamental Research Funds for the Central Universities (KYZ201147); Youth Science and Technology Innovation Fund (KJ2011009); 863 project by Ministry of Science and Technology of China (2011AA100208) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Shengjun Xia, Yu Chen, Jiafu Jiang, Sumei Chen, Zhiyong Guan, Weimin Fang & Fadi Chen

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  1. Shengjun Xia
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  2. Yu Chen
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  3. Jiafu Jiang
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  7. Fadi Chen
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Correspondence to Fadi Chen.

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Shengjun Xia and Yu Chen equally contributed to this study.

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Xia, S., Chen, Y., Jiang, J. et al. Expression profile analysis of genes involved in horizontal gravitropism bending growth in the creeping shoots of ground-cover chrysanthemum by suppression subtractive hybridization. Mol Biol Rep 40, 237–246 (2013). https://doi.org/10.1007/s11033-012-2054-5

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  • DOI: https://doi.org/10.1007/s11033-012-2054-5

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