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Regulation of the major vacuolar Ca2+ transporter genes, by intercellular Ca2+ concentration and abiotic stresses, in tip-burn resistant Brassica oleracea

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Abstract

Calcium is an essential plant macronutrient that has unique structural and signaling roles related to tip-burn disorder in Brassica spp. crops. For two types of cabbage inbred lines, tip-burn susceptible and resistant, we measured and compared major macronutrient cations, including Ca2+, in leaves. In both lines, Ca2+, Mg2+, Na+, and K+, accumulated more in leaf base than in leaf apex. Ca2+ and K+ were >2 times more abundant in the tip-burn resistant line, while Na+ was higher in the susceptible line. Ca2+ differences between the two lines resulted from differential accumulation of calcium into cell vacuoles. We profiled major vacuolar Ca2+ transporters, in both cabbage lines, by growth time and intercellular Ca2+ concentration. Expression pattern of several Ca2+ transporter genes differed between tip-burn susceptible and resistant lines by growth time points. We also identified promoter regions of the major Ca2+ vacuole transporter genes, CAX1, ACA4, and ACA11, which displayed hormonal, light and defense-related cis-acting regulatory elements. Finally, transporter genes in the two cabbage lines responded differently to abiotic stresses, demonstrating diversity in gene regulation among orthologous genes.

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Abbreviations

CAX:

Ca2+/H+ antiporters

ACA:

Ca2+-ATPase

Bo :

Brassica oleracea

At :

Arabidopsis thaliana

LA:

Leaf apex

LM:

Middle of leaf base

LB:

Leaf base

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Acknowledgments

This work was financially supported by grants from the Next-Generation Bio Green 21 Program (No. PJ0082002011) and Cabbage Genomics assisted breeding supporting Center (CGC) research programs and funded by Rural Development Administration and Ministry for Food, Agriculture, Forestry and Fisheries of the Korean Government, respectively.

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Authors and Affiliations

  1. Green Bio Research Center, Cabbage Genomics Assisted Breeding Supporting Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Gwahangno 111, Daejeon, 305-806, South Korea

    Jeongyeo Lee, Inkyu Park, Hong-Seok Park, SeokYoon Kwon & HyeRan Kim

  2. Division of Life Science, Korea Basic Science Institute (KBSI), Daejeon, 305-333, South Korea

    Zee-Won Lee

  3. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Gwahangno 111, Daejeon, 305-806, South Korea

    Suk Weon Kim

  4. Samsung Seeds Company, 128, Madu 2 Gil, Seotan, Pyongtaek, Gyeonggi, 451-851, South Korea

    Namkwon Baek

  5. Department of Crop Science, College of Agriculture & Life Sciences, Chungnam National University, 79 Daehangno, Yuseong-gu, Daejeon, 305-764, Korea

    Sang Un Park

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  1. Jeongyeo Lee
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Correspondence to SeokYoon Kwon or HyeRan Kim.

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Lee, J., Park, I., Lee, ZW. et al. Regulation of the major vacuolar Ca2+ transporter genes, by intercellular Ca2+ concentration and abiotic stresses, in tip-burn resistant Brassica oleracea . Mol Biol Rep 40, 177–188 (2013). https://doi.org/10.1007/s11033-012-2047-4

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