Molecular Biology Reports

, Volume 40, Issue 1, pp 117–127 | Cite as

Cloning and characterization of two rice long-chain base kinase genes and their function in disease resistance and cell death

  • Huijuan Zhang
  • Li Li
  • Yongmei Yu
  • Jibo Mo
  • Lijun Sun
  • Bo Liu
  • Dayong Li
  • Fengming Song


Sphingolipid metabolites such as long-chain base 1-phosphates (LCBPs) have been shown to play an important role in plants; however, little is known about their function in plant disease resistance and programmed cell death (PCD). In the present study, we cloned and identified two rice long-chain base kinase (LCBK) genes (OsLCBK1 and OsLCBK2), which are involved in biosynthesis of LCBPs, and performed functional analysis in transgenic tobacco. Expression of OsLCBK1 and OsLCBK2 was induced in rice seedlings after treatments with defense signaling molecules and after infection by Magnaporthe grisea, the causal agent of blast disease. Transgenic tobacco plants overexpressing OsLCBK1 were generated and disease resistance assays indicate that the OsLCBK1-overexpressing plants showed enhanced disease resistance against Pseudmonassyringae pv. tabacci, the causal agent of wildfire disease, and tobacco mosaic virus. Expression levels of some defense-related genes were constitutively up-regulated and further induced after pathogen infection in the OsLCBK1-overexpressing plants. Treatment with fungal toxin fumonisin B1, an effective inducer of PCD in plants, resulted in reduced level of cell death in the OsLCBK1-overexpressing plants, as indicated by cell death staining, leakage of electrolyte and expression of hypersensitive response indicator genes. These data suggest that rice LCBKs, probably through regulation of endogenous LCBP level, play important roles in disease resistance response and PCD in plants.


Rice (Oryza sativa L.) Long-chain base 1-phosphate (LCBP) Long-chain base kinase (LCBK) Transgenic tobacco Disease resistance Programmed cell death 



Abscisic acid


1-Amino cyclopropane-1-carboxylic acid








Fumonisin B1


Hypersensitive response


Jasmonic acid


Long-chain base


Long-chain base kinase


Long-chain base 1-phosphate


Programed cell death


Pseudomonas syringae pv. tobacci


Reverse transcriptase-polymerase chain reaction




Salicylic acid


Sphingosine kinase


Tobacco mosaic virus


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Huijuan Zhang
    • 1
  • Li Li
    • 1
  • Yongmei Yu
    • 1
  • Jibo Mo
    • 1
  • Lijun Sun
    • 1
  • Bo Liu
    • 1
  • Dayong Li
    • 1
  • Fengming Song
    • 1
    • 2
  1. 1.State Key Laboratory for Rice Biology, Institute of BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of Plant Protection, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China

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