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Characterization of ascorbate peroxidase in soybean under flooding and drought stresses

Molecular Biology Reports volume 39, pages 10573–10579 (2012)Cite this article

Abstract

Flooding and drought are the two different forms of water stress that adversely affect the growth and development of soybean plant in particular at early stage. Ascorbate peroxidase (APX) is a known antioxidant enzyme that plays key role in abiotic stresses. To investigate the changes in APX in soybean under drought and flooding stresses, western blotting, enzyme activity assay and biophoton emission techniques were used. Flooding stress was imposed by adding excess amount of water in the sand and drought by withholding water supply. Under flooding stress, a decrease in APX was detected with time. Completely opposite trend was evident in hypocotyl and root of plants exposed to drought. Western blotting and APX activity results are complementary to each other. Biophoton emissions further confirmed the increasing and decreasing trend of APX under drought and flooding stress, respectively.

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Abbreviations

APX:

Ascorbate peroxidase

ROS:

Reactive oxygen species

CBB:

Coomassie brilliant blue

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Acknowledgments

The authors thank Dr. Yohei Nanjo and Dr. Keito Nishizawa at National Institute of Crop Science for their valuable comments and discussion. We also thank Higher Education Commission of Pakistan for awarding International Research Support Initiative Scholarship grant. We thankfully acknowledge the financial support provided through the DST-BOYSCAST fellowship programme, Government of India. We also acknowledge National Institute of Crop Science, Japan for providing the research facilities. This work was supported by a Grant-in-Aid for Challenging Exploratory Research (23658063) from Japan Society for the Promotion of Science.

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Affiliations

  1. National Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan

    Rehana Kausar, Zahed Hossain & Setsuko Komatsu

  2. Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, Pakistan

    Rehana Kausar

  3. Department of Botany, West Bengal State University, Kolkata, 700126, West Bengal, India

    Zahed Hossain

  4. Graduate School for the Creation of New Photonics Industries, Hamamatsu, 431-1202, Japan

    Takahiro Makino

Authors
  1. Rehana Kausar
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  2. Zahed Hossain
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  3. Takahiro Makino
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  4. Setsuko Komatsu
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Corresponding author

Correspondence to Setsuko Komatsu.

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Kausar, R., Hossain, Z., Makino, T. et al. Characterization of ascorbate peroxidase in soybean under flooding and drought stresses. Mol Biol Rep 39, 10573–10579 (2012). https://doi.org/10.1007/s11033-012-1945-9

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  • DOI: https://doi.org/10.1007/s11033-012-1945-9

Keywords

  • Soybean
  • Flooding
  • Drought
  • Ascorbate peroxidase
  • Biophoton