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Molecular characterization of differentially expressed TXNIP gene and its association with porcine carcass traits

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Abstract

Thioredoxin interacting protein (TXNIP), which plays a regulatory role in lipid metabolism and immune regulation, is down-regulated expressed in F1 hybrids Landrace × Yorkshire skeletal muscle. Here we described the molecular characterization of porcine TXNIP gene. The full-length cDNA contains a coding sequence of 1,176 bp nucleotides with untranslated regions of 263 bp at 5′-end and 441 bp at 3′-end, respectively. The predicted molecular mass and isoelectric point of porcine TXNIP is 43.81 kDa and 7.385, respectively. The deduced 391 amino acids exhibit high identity with other mammalian TXNIP. The TXNIP gene contains eight coding exons and seven non coding introns, spans approximately 3,348 bp. The expression of porcine TXNIP mRNA is almost absent in Landrace × Yorkshire and lower level in 6-month-old pigs during skeletal muscle development. Other stages and breeds were high level expressed. Statistical analysis showed the TXNIP gene polymorphism (c.575-4T>C) was different between F1 hybrids and their parents, was highly associated with dressing percentage (DP) and thorax–waist fat thickness (TFT) in the Yorkshire × Meishan F2 population. The possible role of TXNIP was discussed.

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Acknowledgments

The project was supported by Agricultural Science Innovation Foundation of Hubei Province, China (2007-620), the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20090146120030), National Natural Science Foundation of China (31201764, 31172179) and Key Program of National Transgenic Research of China (Grant No. 2009ZX08009-161B & 2009ZX08009-151B).

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Authors and Affiliations

  1. Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China

    Min Liu, Xianfeng Ling, Yuanzhu Xiong & Dequan Xu

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  1. Min Liu
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  2. Xianfeng Ling
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  3. Yuanzhu Xiong
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  4. Dequan Xu
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Correspondence to Dequan Xu.

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Liu, M., Ling, X., Xiong, Y. et al. Molecular characterization of differentially expressed TXNIP gene and its association with porcine carcass traits. Mol Biol Rep 39, 10439–10446 (2012). https://doi.org/10.1007/s11033-012-1923-2

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  • DOI: https://doi.org/10.1007/s11033-012-1923-2

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