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Association of the C242T polymorphism in the NADPH oxidase p22 phox gene with carotid atherosclerosis in Slovenian patients with type 2 diabetes

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Abstract

Oxidative stress plays an important role in the pathogenesis of diabetes and its complications. Genetic variations of enzymes producing reactive oxygen species could change their activity, thus contributing to the susceptibility to oxidative stress. The aim of this study was to examine the role of the NADPH oxidase C242T polymorphism in the development of carotid atherosclerosis in patients with type 2 diabetes. 286 diabetic patients and 150 healthy controls were enrolled in the study. Carotid atherosclerosis was quantified ultrasonographically as carotid intima-media thickness, plaque score (0–6) and plaque type (1–5). Diabetic patients were divided into low and high risk groups based on ultrasound phenotypes of carotid atherosclerosis. Genotypes were determined by real-time PCR. Levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) were measured by enzyme-linked immunosorbent assay (ELISA). Diabetic patients demonstrated a statistically significant difference compared to healthy controls in the following parameters: age, BMI, waist circumference, smoking prevalence, glucose, triglyceride and 8-OHdG serum levels. Control subjects had significantly higher levels of HDL, LDL and total cholesterol than diabetics (p < 0.001). The NADPH C242T polymorphism was not related with clinical characteristics, lipid parameters and 8-OHdG serum levels. We found no significant difference in the NADPH genotype distribution between diabetics and controls (p = 0.19) nor between low and high risk subgroups of diabetics (mean CIMT: p = 0.67; plaque score: p = 0.49, plaque type: p = 0.56). In the present study the NADPH C242T polymorphism was not associated with the degree of oxidative stress and carotid atherosclerosis. Further studies will show if it can be used as a genetic marker for carotid atherosclerosis in diabetic patients.

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Acknowledgments

The authors thank Mrs. Brina Beškovnik Hrastar, BA, for revising the English.

Conflict of interest

The authors declare no conflict of interest related to this work.

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Authors and Affiliations

  1. General Hospital Rakičan, Ulica dr. Vrbnjaka 6, 9000, Murska Sobota, Slovenia

    Marija Šantl Letonja

  2. Medical Faculty, Institute of Histology and Embriology, University of Ljubljana, Vrazov trg 2, 1105, Ljubljana, Slovenia

    Jovana Nikolajević-Starčević & Danijel Petrovič

  3. Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), University of Porto, Largo Prof. Abel Salazar, 2, 4099-003, Porto, Portugal

    Daniela Cristina Branco Batista

  4. Clinical Institute of Clinical Chemistry and Clinical Biochemistry, University Medical Center, Ljubljana, Slovenia

    Joško Osredkar

  5. Medical Faculty of Ljubljana, Institute of Histology and Embriology, University Ljubljana, Korytkova 2, 1105, Ljubljana, Slovenia

    Danijel Petrovič

Authors
  1. Marija Šantl Letonja
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  2. Jovana Nikolajević-Starčević
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Correspondence to Danijel Petrovič.

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Marija Šantl Letonja, and Jovana Nikolajević-Starčević are contributed equally.

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Letonja, M.Š., Nikolajević-Starčević, J., Batista, D.C.B. et al. Association of the C242T polymorphism in the NADPH oxidase p22 phox gene with carotid atherosclerosis in Slovenian patients with type 2 diabetes. Mol Biol Rep 39, 10121–10130 (2012). https://doi.org/10.1007/s11033-012-1886-3

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  • DOI: https://doi.org/10.1007/s11033-012-1886-3

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