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Differential responses of peripheral circadian clocks to a short-term feeding stimulus

Molecular Biology Reports volume 39, pages 9783–9789 (2012)Cite this article

Abstract

To investigate the effects of a short-term feeding stimulus on the expression of circadian genes in peripheral tissues, we examined the effects of a 30-min feeding stimulus on the rapid responses and circadian phases of five clock genes (Bmal1, Cry1, Per1, Per2 and Per3) and a clock-controlled gene (Dbp) in the heart and kidney of rats. A 30 min feeding stimulus was sufficient to alter the transcript levels and circadian phases of peripheral clock genes in a tissue-specific manner. The transcript levels of most clock genes (Bmal1, Cry1, Per1, and Per2) were significantly down-regulated in the heart within 2 h, which were affected marginally in the kidney (except Per1). In addition to the rapid response of clock gene expression, we found that the circadian phases of these clock genes were markedly shifted by the 30-min feeding stimulus in the heart within 1 day. However, the same feeding stimulus almost not affected the peak phases of these clock genes in the kidney. Moreover, these differential responses of peripheral clocks to the 30-min feeding were also similarly reflected in the expression of circadian output gene Dbp. Therefore, a 30-min feeding stimulus was sufficient to induce dyssynchronized peripheral circadian rhythm and might further result in disordered downstream physiological function in rats.

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Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (no. 30970364), the Natural Science Foundation of Zhejiang Province, China (no. Y3090563), the Program for Changjiang Scholars and Innovative Research Team in University (no. IRT 0653).

Conflict of interest

The authors report no conflicts of interest.

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Affiliations

  1. College of Biological and Environmental Engineering, Zhejiang University of Technology, No. 6 District, Zhaohui, Hangzhou, Zhejiang, 310032, China

    Tao Wu, Ling Yao, Lu Sun, Fen ZhuGe & Zhengwei Fu

  2. College of Animal Sciences, Zhejiang University, Hangzhou, China

    Ou Fu

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  1. Tao Wu
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  2. Ou Fu
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  3. Ling Yao
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  4. Lu Sun
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  5. Fen ZhuGe
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  6. Zhengwei Fu
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Correspondence to Zhengwei Fu.

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Tao Wu and Ou Fu have equally contributed to this work.

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Wu, T., Fu, O., Yao, L. et al. Differential responses of peripheral circadian clocks to a short-term feeding stimulus. Mol Biol Rep 39, 9783–9789 (2012). https://doi.org/10.1007/s11033-012-1844-0

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  • DOI: https://doi.org/10.1007/s11033-012-1844-0

Keywords

  • Short-term feeding
  • Peripheral clocks
  • Clock genes
  • Kidney
  • Heart