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Transcriptional responses to drought stress in root and leaf of chickpea seedling

Molecular Biology Reports volume 39, pages 8147–8158 (2012)Cite this article

Abstract

Chickpea (Cicer arietinum L.) is an important pulse crop grown mainly in the arid and semi-arid regions of the world. Due to its taxonomic proximity with the model legume Medicago truncatula and its ability to grow in arid soil, chickpea has its unique advantage to understand how plant responds to drought stress. In this study, an oligonucleotide microarray was used for analyzing the transcriptomic profiles of unigenes in leaf and root of chickpea seedling under drought stress, respectively. Microarray data showed that 4,815 differentially expressed unigenes were either ≥2-fold up- or ≤0.5-fold down-regulated in at least one of the five time points during drought stress. 2,623 and 3,969 unigenes were time-dependent differentially expressed in root and leaf, respectively. 110 pathways in two tissues were found to respond to drought stress. Compared to control, 88 and 52 unigenes were expressed only in drought-stressed root and leaf, respectively, while nine unigenes were expressed in both the tissues. 1,922 function-unknown unigenes were found to be remarkably regulated by drought stress. The expression profiles of these time-dependent differentially expressed unigenes were useful in furthering our knowledge of molecular mechanism of plant in response to drought stress.

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Acknowledgments

We gratefully acknowledge the partial financial support from the projects supported by the National Natural Science Foundation of China (30960201, 30960206, 30860152 and 31160306), from the project supported by the Xinjiang Science and Technology Department of China (200991254), from the projects supported by the National Science and Technology Ministry (2006BAD09A04, 2006BAD09A08), from the project supported by the National Science Foundation for Postdoctoral Scientists of China (20080431107), from the project supported by the Jiangsu Science Foundation of Postdoctoral Scientists of China (0801048B) for this research.

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Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Xiansheng Wang, Yuying Jia, Hanyan Gu, Hongyu Ma, Tian Yu & Hao Ma

  2. College of Agriculture, Hebei University of Engineering, Hebei, 056038, China

    Ying Liu

  3. Key Laboratory of Agriculture Biotechnology, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China

    Hua Zhang, Quanjia Chen, Lin Ma, Aixing Gu, Jusong Zhang & Shubing Shi

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  1. Xiansheng Wang
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  2. Ying Liu
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  3. Yuying Jia
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  10. Aixing Gu
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  13. Hao Ma
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Corresponding authors

Correspondence to Shubing Shi or Hao Ma.

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Xiansheng Wang and Ying Liu contributed equally to this work.

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Wang, X., Liu, Y., Jia, Y. et al. Transcriptional responses to drought stress in root and leaf of chickpea seedling. Mol Biol Rep 39, 8147–8158 (2012). https://doi.org/10.1007/s11033-012-1662-4

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  • DOI: https://doi.org/10.1007/s11033-012-1662-4

Keywords

  • Chickpea
  • Oligonucleotide microarray
  • Drought
  • Transcriptional response
  • Root
  • Leaf