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Identification and characterization of a novel calcyclin binding protein (CacyBP) gene from Apis cerana cerana

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Abstract

Calcyclin binding protein (CacyBP), a homolog of Sgt1, was shown to interact with some S100 proteins, Skp1, tubulin, actin and ERK1/2 kinases. Studies have also shown that CacyBP is a neuronal protein in mammals. Limited information is available regarding the properties and functions of CacyBP in insects. Here, we cloned and characterized a novel CacyBP gene, named AccCacyBP, from honeybee (Apis cerana cerana). Bioinformatic analysis indicated that AccCacyBP was highly conserved and closely related to the CacyBP of other insects. Promoter analysis revealed a number of putative tissue, development and stress-related transcription factor-binding sites. RT-qPCR demonstrated that AccCacyBP was expressed at all of the stages of development, especially in the brains of honeybees. Moreover, immunohistochemistry analysis showed the presence of AccCacyBP in the brain. The transcript levels of AccCacyBP in the brains of honeybees were developmentally induced and upregulated by exposure to oxidative stresses, including UV-light, acetamiprid and HgCl2. This study demonstrates that the CacyBP gene in honeybees may be a neuronal protein involved in the developmental regulation and the stress-response of the brain of honeybees.

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Acknowledgments

This work was financially supported by the China Agriculture Research System (No. CARS-45), the Agro-scientific Research in the Public Interest (No. 200903006) and the National Natural Science Foundation (No. 31172275) in China.

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Authors and Affiliations

  1. College of Life Sciences, Shandong Agricultural University, Taian, 271018, Shandong, People’s Republic of China

    Xiaoli Yu, Wenjing Lu & Xingqi Guo

  2. College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, People’s Republic of China

    Rujiang Sun & Baohua Xu

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  1. Xiaoli Yu
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  2. Wenjing Lu
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  4. Xingqi Guo
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Correspondence to Xingqi Guo or Baohua Xu.

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11033_2012_1652_MOESM1_ESM.tif

Fig. 1: Properties and sequence analysis of AccCacyBP. A: The comparison of the deduced amino acid sequence of Apis cerana cerana calcyclin binding protein (AccCacyBP) with its homologs: AmelCacyBP (Apis mellifera, XP396161), CfCacyBP (Camponotus floridanus, EFN69732), AeCacyBP (Acromyrmex echinatior, EGI62763), HsCacyBP (Homo sapiens, AAH67823), RnCacyBP (Rattus norvegicus, NP001004208). Identical or conserved amino acids are shaded in black or gray, respectively. The predicted domains are shown with letters (A, B and C) on the top of the sequences. Schematic representation of AccCacyBP indicates the binding sites of its ligands and numbers indicate the position of amino acid residues. B: The phylogenetic relationships of AccCacyBP and other CacyBP proteins. Numbers above or below branch nodes represent the confidence level of posterior probability. The sequences used are as follows: DrCacyBP (Danio rerio, NP998052), IfCacyBP (Ictalurus furcatus, ADO28075), SsCacyBP (Salmo salar, ACN10387), CfCacyBP (Camponotus floridanus, EFN69732), AmelCacyBP (Apis mellifera, XP396161), AeCacyBP (Acromyrmex echinatior, EGI62763), HsaCacyBP (Harpegnathos saltator, EFN81395), CqCacyBP (Culex quinquefasciatus, XP001868830), MmCacyBP (Mus musculus, NP033916), RnCacyBP (Rattus norvegicus, NP001004208), HsCacyBP (Homo sapiens, AAH67823), AtCacyBP (Arabidopsis thaliana, NP564346), ElCacyBP (Esox lucius, ACO13192). (TIFF 5968 kb)

11033_2012_1652_MOESM2_ESM.tif

Fig. 2: The nucleotide sequence and putative transcription factor-binding sites of the 5′-flanking region of AccCacyBP. The translation (ATG) and transcription start sites are marked with arrows. The transcription factor-binding sites are boxed. (TIFF 2964 kb)

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Yu, X., Lu, W., Sun, R. et al. Identification and characterization of a novel calcyclin binding protein (CacyBP) gene from Apis cerana cerana . Mol Biol Rep 39, 8053–8063 (2012). https://doi.org/10.1007/s11033-012-1652-6

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