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Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen

Abstract

Two aromatic rice genotypes, Pusa Basmati 1 (PB1) and Kalanamak 3119 (KN3119) having 120 and 30 kg/ha optimum nitrogen requirement respectively, to produce optimal yield, were chosen to understand their differential nitrogen responsiveness. Both the genotypes grown under increasing nitrogen inputs showed differences in seed/panicle, 1,000 seed weight, %nitrogen in the biomass and protein content in the seeds. All these parameters in PB1 were found to be in the increasing order in contrast to KN3119 which showed declined response on increasing nitrogen dose exceeding the normal dose indicating that both the genotypes respond differentially to the nitrogen inputs. Gene expression analysis of members of ammonium transporter gene family in flag leaves during active grain filling stage revealed that all the three members of OsAMT3 family genes (OsAMT1;1–3), only one member of OsAMT2 family i.e., OsAMT2;3 and the high affinity OsAMT1;1 were differentially expressed and were affected by different doses of nitrogen. In both the genotypes, both increase and decline in seed protein contents matched with the expressions levels of OsAMT1;1, OsGS1;1 and OsGS1;2 in the flag leaves during grain filling stage indicating that high nitrogen nutrition in KN3119 probably causes the repression of these genes which might be important during grain filling.

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References

  1. 1.

    Camanes G, Cerezo M, Primo-Millo E, Gojon A, Garcıa-Agustın P (2007) Ammonium transport and CitAMT1 expression are regulated by light and sucrose in Citrus plants. J Exp Bot 58(11):2811–2825

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    Loque′ D, von Wire′n N (2004) Regulatory levels for the transport of ammonium in plant roots. J Exp Bot 55:1293–1305

    Article  Google Scholar 

  3. 3.

    Gazzarrini S, Lejay T, Gojon A, Ninnemann O, Frommer WB, von Wire′n N (1999) Three functional transporters for constitutive, diurnally regulated, and starvation-induced uptake of ammonium into Arabidopsis roots. Plant Cell 11:937–947

    PubMed  CAS  Google Scholar 

  4. 4.

    Aslam M, Travis RL, Rains DW, Huffaker RC (1996) Effect of ammonium on the regulation of nitrate and nitrite transport systems in roots of intact barley (Hordeum vulgare L.) seedlings. Planta 200:58–63

    Article  CAS  Google Scholar 

  5. 5.

    Ninnemann O, Jauniaux JC, Frommer WB (1994) Identification of a high-affinity NH4 + transporter from plants. EMBO J 13:3464–3471

    PubMed  CAS  Google Scholar 

  6. 6.

    Pearson CJ, Finnegan PM, Schjoerring JK (2002) Regulation of the high-affinity ammonium transporter (BnAMT1;2) in the leaves of Brassica napus by nitrogen status. Plant Mol Biol 49:483–490

    PubMed  Article  CAS  Google Scholar 

  7. 7.

    von WireÂn N, Gazzarrini S, Gojon A, Frommer WB (2000) The molecular physiology of ammonium uptake and retrieval. Curr Opin Plant Biol 3:254–261

    Google Scholar 

  8. 8.

    Lauter FR, Ninnemann O, Bucher M, Riesmeier JW, Frommer WB (1996) Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato. Proc Nat Acad Sci USA 93:8139–8144

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Wang MY, Glass ADM, Shaff JE, Kochian LV (1994) Ammonium uptake by rice roots. III. Electrophysiology. Plant Physiol 104:899–906

    PubMed  CAS  Google Scholar 

  10. 10.

    Suenaga A, Sonoda MK, Ikeda Y, von Wirén A, Hayakawa N, Yamaguchi T, Yamaya T (2003) Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants. Plant Cell Physiol 44(2):206–211

    PubMed  Article  CAS  Google Scholar 

  11. 11.

    Sonoda Y, Ikeda A, Saiki S, von Wirén N, Yamaya T, Yamaguchi J (2003) Distinct expression and function of three ammonium transporter genes (OsAMT1;1–1;3) in rice. Plant Cell Physiol 44(7):726–734

    PubMed  Article  CAS  Google Scholar 

  12. 12.

    Kronzucker HJ, Schjoerring JK, Erner Y, Kirk GJD, Siddiqi MY, Glass ADM (1998) Dynamic interactions between root NH4 + influx and long-distance N translocation in rice: insights into feedback processes. Plant Cell Physiol 39:1287–1293

    Article  CAS  Google Scholar 

  13. 13.

    Yamaya T, Oaks A (2004) Metabolic regulation of ammonium uptake and assimilation. In: Amaˆncio S, Stulen I (eds) Nitrogen acquisition and assimilation in higher plants. Kluwer Academic Publishers, Dordrecht/Boston/London, pp 35–63

    Chapter  Google Scholar 

  14. 14.

    Peoples MB, Gifford RM (1993) Long-distance transport of carbon and nitrogen from sources to sinks in higher plants. In: Dennis DT, Turpin DH (eds) Plant physiology, biochemistry and molecular biology. Wiley, New York, pp 434–447

    Google Scholar 

  15. 15.

    Lea PJ (1993) Nitrogen metabolism. In: Lea PJ, Leegood RC (eds) Plant biochemistry and molecular biology. Wiley, New York, pp 155–180

    Google Scholar 

  16. 16.

    Kamachi K, Yamaya T, Mae T, Ojima K (1991) A role for glutamine synthetase in the remobilization of leaf nitrogen during natural senescence in rice leaves. Plant Physiol 96:411–417

    PubMed  Article  CAS  Google Scholar 

  17. 17.

    Tabuchi M, Sugiyama K, Ishiyama K, Inoue E, Sato T, Takahashi H, Yamaya T (2005) Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1. Plant J 42:641–651

    PubMed  Article  CAS  Google Scholar 

  18. 18.

    Livak KJ, Schmitten TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta c(T)) method. Methods 25:402–408

    PubMed  Article  CAS  Google Scholar 

  19. 19.

    Subbaiah BV, Asija CL (1956) A rapid procedure for the determination of available nitrogen in soils. Curr Sci 25:259–262

    Google Scholar 

  20. 20.

    Kumar R, Taware R, Gaur VS, Guru SK, Kumar A (2009) Influence of nitrogen on the expression of TaDof1 transcription factor in wheat and its relationship with photosynthetic and ammonium assimilating efficiency. Mol Biol Rep 36(8):2209–2220

    PubMed  Article  CAS  Google Scholar 

  21. 21.

    Singh RK, Singh US, Khush GS, Rohilla R (2000) Genetics and biotechnology quality traits in aromatic rices. In: Singh RK, Singh US, Khush GS (eds) Aromatic Rices. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi, pp 47–69

    Google Scholar 

  22. 22.

    Manzoor Z, Awan TH, Safdar ME, Ali RI, Ashraf MM, Ahmad M (2006) Effect of nitrogen levels on yield and yield components of basmati 2000. J Agri Res 44(2):115–122

    Google Scholar 

  23. 23.

    Gaur VS, Singh US, Gupta AK and Kumar A. 2011. Understanding the differential nitrogen sensing mechanism in rice genotypes through expression analysis of high and low affinity ammonium transporter genes. Accepted in Molecular Biology Reports. doi: 10.1007/s11033-011-0972-2

  24. 24.

    Kentaro H, Syuntaro H, Satoru I, Isamu N (2008) Ammonia exchange between rice leaf blades and the atmosphere: effect of broadcast urea and changes in xylem sap and leaf apoplastic ammonium concentrations. Soil Sci Plant Nutr 54:807–818

    Article  Google Scholar 

  25. 25.

    Kaiser BN, Rawat SR, Siddiqi MY, Masle J, Glass ADM (2002) Functional analysis of an Arabidopsis T-DNA `Knockout’ of the high-affnity NH4 + transporter AtAMT1;1. Plant Physiol 130:1263–1275

    PubMed  Article  CAS  Google Scholar 

  26. 26.

    Tabuchi M, Abiko T, Yamaya T (2007) Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.). J Exp Bot 58(9):2319–2327

    PubMed  Article  CAS  Google Scholar 

  27. 27.

    Sun GR, Zhu P, Liu WF, Xiao YH (1994) Glutamine synthetase activity and rice heterosis prediction. J Wuhan Bot Res 12(2):149–153

    Google Scholar 

  28. 28.

    Zhu HM, Rong XM, Liu Q, Peng JW (2001) Differences in contents of grain protein of different genotype rice varieties. J Hunan Agric Univ Nat Sci 27(1):13–16

    CAS  Google Scholar 

  29. 29.

    Tang XR, Guan CY, Yu TQ (1999) Studies on matter metabolism of yield and quality of different genotypic rice. J Hunan Agric Univ 25(4):279–282

    CAS  Google Scholar 

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Acknowledgments

The present investigation was a part of the DBT (Department of Biotechnology), Govt. of India supported JRF programme. Financial assistance provided by DBT to Vikram Singh Gaur and Alok Kumar Gupta is duly acknowledged.

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Correspondence to Anil Kumar.

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Gaur, V.S., Singh, U.S., Gupta, A.K. et al. Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen. Mol Biol Rep 39, 8035–8044 (2012). https://doi.org/10.1007/s11033-012-1650-8

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Keywords

  • Ammonium transporter
  • Nitrogen use efficiency
  • Gene expression