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Association study of interleukin-4 polymorphisms with paranoid schizophrenia in the Polish population: a critical approach

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Abstract

Changes in immunological system are one of dysfunctions reported in schizophrenia. Some changes based on an imbalance between Th1 and Th2 cytokines results from cytokine gene polymorphisms. Interleukin-4 gene (IL4) is considered as a potential candidate gene in schizophrenia association studies. The aim of the current case–control study was to examine whether the −590C/T (rs2243250) and −33C/T (rs2070874) IL4 gene polymorphisms are implicated in paranoid schizophrenia development in the Polish population. Genotyping of polymorphisms was performed by using PCR–RFLP technique. The genotypes and alleles distribution of both SNPs were analysed in patients (n = 182) and healthy individuals constituted the control group (n = 215). The connection between some clinical variables and studied polymorphisms has been examined as well. We did not revealed any association between the −590C/T and −33C/T polymorphisms and paranoid schizophrenia. In case of both SNPs the homozygous TT genotype was extremely rare. Both polymorphic sites of the IL4 gene were found to be in a very strong linkage disequilibrium. However we did not identify a haplotype predispose to paranoid schizophrenia. No associations were also observed between the clinical course and psychopathology of the disease and the genotypes of both analysed polymorphisms. Our results suggest that the polymorphisms −590C/T in IL4 gene promoter region and −33C/T in the 5′-UTR are not involved in the pathophysiology of paranoid schizophrenia in Polish residents.

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Acknowledgments

This study was funded by the grant (No. KNW-1-134/10) from the Medical University of Silesia in Katowice, Poland.

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Authors and Affiliations

  1. Department of Medical Genetics, Medical University of Silesia, Ostrogorska 30, 41-200, Sosnowiec, Poland

    Anna Fila-Danilow, Malgorzata Kowalczyk, Monika Paul-Samojedny, Paulina Borkowska, Renata Suchanek & Jan Kowalski

  2. Department of Psychiatry and Psychotherapy, Medical University of Silesia, Ziolowa 45/47, 40-635, Katowice, Poland

    Krzysztof Kucia

  3. Division of Statistics, Department of Instrumental Analysis, Medical University of Silesia, Ostrogorska 30, 41-200, Sosnowiec, Poland

    Aleksander Owczarek

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  1. Anna Fila-Danilow
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  2. Krzysztof Kucia
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  4. Aleksander Owczarek
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Correspondence to Anna Fila-Danilow.

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Fila-Danilow, A., Kucia, K., Kowalczyk, M. et al. Association study of interleukin-4 polymorphisms with paranoid schizophrenia in the Polish population: a critical approach. Mol Biol Rep 39, 7941–7947 (2012). https://doi.org/10.1007/s11033-012-1639-3

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  • DOI: https://doi.org/10.1007/s11033-012-1639-3

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