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Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs

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Abstract

IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.

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Acknowledgment

The study was supported by Program for Changkiang Scholars and Innovative Research Team in University (IRTO555) and the earmarked fund for Modern Agro-industry Technology Research System of China (CARS-36). The authors would like to thank the personnel of these teams for their kind assistance.

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Authors and Affiliations

  1. Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan, 625014, People’s Republic of China

    Ye Zhao, Bing Yu, XiangBing Mao, Qian Mao, Zhiqing Huang & Daiwen Chen

  2. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Ya’an, Sichuan, 625014, People’s Republic of China

    Ye Zhao, Bing Yu, GuoQuan Han, Qian Mao & Daiwen Chen

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  1. Ye Zhao
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  2. Bing Yu
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  3. XiangBing Mao
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  5. Qian Mao
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  6. Zhiqing Huang
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Correspondence to Daiwen Chen.

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Zhao, Y., Yu, B., Mao, X. et al. Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs. Mol Biol Rep 39, 7011–7017 (2012). https://doi.org/10.1007/s11033-012-1531-1

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  • DOI: https://doi.org/10.1007/s11033-012-1531-1

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