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Complete mitogenome sequence of black carp (Mylopharyngodon piceus) and its use for molecular phylogeny of leuciscine fishes

Molecular Biology Reports volume 39, pages 6337–6342 (2012)Cite this article

Abstract

The black carp Mylopharyngodon piceus (Cyprinidae), native to eastern Asian, is a large, commercially important fish, and has been introduced to many other countries for variable reasons. In this study, the complete mitochondrial genome sequences from three specimens of black carp were first determined and were used to evaluate the sister relationship between black carp and grass carp (Ctenopharyngodon idellus). Two individuals had a mitogenome of 16,609 bp, while the other was 16,611 bp in length. Similar to most vertebrates, the black carp contains the same gene order and an identical number of genes or regions, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 putative control region. Phylogenetic analyses using three different computational algorithms (Maximum Parsimony, Maximum Likelihood, and Bayesian analysis) revealed two distinct clades in subfamily Leuciscinae. However, the sister taxonomic relationship of black carp and grass carp was not observed using sequences of nearly complete mitochondrial genomes, which suggests more nuclear gene markers are needed to resolve the phylogenetic relationship between black carp and grass carp.

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Acknowledgments

The authors are grateful to Minghu Tang and Lixia Fu for their help with fish collections. Meanwhile, the authors want to express special thanks to the reviewers for their valuably constructive comments. The work was supported by the National Natural Science Foundation of China (Grant No. 30630051) and the Shanghai Leading Academic Discipline project (Grant No. Y1101).

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Affiliations

  1. Key Laboratory of Aquatic Genetic Resources and Utilization Certificated by Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China

    Chenghui Wang, Jun Wang, Jinquan Yang, Xiao Song, Qin Chen, Jiawei Xu, Qinling Yang & Sifa Li

  2. Department of Biology, University of Nebraska at Omaha, Omaha, NE, 68182, USA

    Guoqing Lu

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  1. Chenghui Wang
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  2. Jun Wang
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Correspondence to Chenghui Wang or Guoqing Lu.

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Wang, C., Wang, J., Yang, J. et al. Complete mitogenome sequence of black carp (Mylopharyngodon piceus) and its use for molecular phylogeny of leuciscine fishes. Mol Biol Rep 39, 6337–6342 (2012). https://doi.org/10.1007/s11033-012-1455-9

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  • DOI: https://doi.org/10.1007/s11033-012-1455-9

Keywords

  • Mylopharyngodon piceus
  • Mitochondrial genome
  • Leuciscinae
  • Phylogeny