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Common polymorphisms in CYP1A1, GSTM1, GSTT1, GSTP1 and XPD genes and endogenous DNA damage

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Abstract

Endogenous DNA damage levels were analyzed in relation to polymorphisms in genes encoding phase I detoxifying enzyme—CYP1A1, phase II detoxifying enzymes—GSTM1, GSTT1, GSTP1 and enzyme involved in nucleotide excision repair-XPD. The study group consisted of 220 healthy non-smoking volunteers; 90 men and 130 woman, 25–60 years old (44 ± 10 years). The level of DNA damage (% DNA in tail) was evaluated by alkaline comet assay. The genetic variants were determined by restriction fragment length polymorphism PCR. The highest level of DNA damage (6.7%) was found in carriers of both: AA variant of XPD gene and M1 null variant of GSTM1 gene. The lowest level of DNA breaks (3.7%) was associated with the genotype GSTP1-AA/GSTM1 (+).

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Acknowledgment

This work was supported by grant number N404042/32/0945 from the Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Department of Nutrigenomics, National Food and Nutrition Institute, Warsaw, Poland

    Marta Wlodarczyk & Grazyna Nowicka

  2. Department of Pharmacogenomics, Medical University of Warsaw, Warsaw, Poland

    Grazyna Nowicka

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  1. Marta Wlodarczyk
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  2. Grazyna Nowicka
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Correspondence to Marta Wlodarczyk.

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Wlodarczyk, M., Nowicka, G. Common polymorphisms in CYP1A1, GSTM1, GSTT1, GSTP1 and XPD genes and endogenous DNA damage. Mol Biol Rep 39, 5699–5704 (2012). https://doi.org/10.1007/s11033-011-1378-x

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  • DOI: https://doi.org/10.1007/s11033-011-1378-x

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