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Identification and expression analysis of two splice variants of the 14-3-3 epsilon from Litopenaeus Vannamei during WSSV infections

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Abstract

The 14-3-3 epsilon (14-3-3ε) is a member of the 14-3-3-protein family claimed to play important roles in many biological processes. In this study, two alternative 14-3-3 epsilon mRNAs, designated as 14-3-3EL and 14-3-3ES were identified from the shrimp L. vannamei. The 14-3-3EL isoform contains an insertion of 48 nucleotides by intron retention in the pre-mRNA of 14-3-3ε. While the 14-3-3ES occurred after being fully spliced. Using the yeast two hybrid method, the pattern of dimer formation by the two alternative 14-3-3ε isoforms revealed that the shrimp 14-3-3ε formed both homodimers and heterodimers. Both 14-3-3ε transcript variants were constitutively expressed in all shrimp tissues tested but the level of the 14-3-3ES isoform was always lower. However, after white spot syndrome virus (WSSV) infection, the expression level of the two transcript variants changed. At 48 h after infection, expression of 14-3-3EL mRNA increased significantly in the gill and muscle tissue whereas the expression 14-3-3ES increased only in muscle. It was of interest that in the lymphoid organ, there was a significant down-expression of both transcript variants. From these results we suggest that 14-3-3EL and 14-3-3ES might be related to different cellular processes that are modulated during virus infection.

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Acknowledgments

This work was supported by grants from The Thailand Research Fund (TRF) and The Commission on Higher Education (CHE) to Ms. Warapond Wanna (RMU5480007) and the Thailand Government Research Fund (RA1-2551-010 and RA-2553-02-010) from Faculty of Sciences, Prince of Songkla University. We thank Dr. Brian Hodgson for checking the manuscript and valuable comments.

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Authors and Affiliations

  1. Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand

    Warapond Wanna & Amornrat Phongdara

  2. Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla, Thailand

    Jaturon Thipwong & Wiparat Mahakaew

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  1. Warapond Wanna
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  2. Jaturon Thipwong
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Correspondence to Warapond Wanna.

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Wanna, W., Thipwong, J., Mahakaew, W. et al. Identification and expression analysis of two splice variants of the 14-3-3 epsilon from Litopenaeus Vannamei during WSSV infections. Mol Biol Rep 39, 5487–5493 (2012). https://doi.org/10.1007/s11033-011-1351-8

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  • DOI: https://doi.org/10.1007/s11033-011-1351-8

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