Abstract
Nasal polyps are strongly associated with a risk of chronic rhinosinusitis development as well as other obstruction including asthma and allergy. The following study tested the association of the 140A/G polymorphism of lactoferine (LF) encoding gene and the −33C/G polymorphism of osteoblast-specific factor-2 (OSF-2) encoding gene with a risk of chronic rhinosinusitis with nasal polyps in a Polish population. One hundred ninety five patients of chronic rhinosinusitis with nasal polyps as well as 200 sex, age and ethnicity matched control subjects without chronic sinusitis and nasal polyps were enrolled in this study. Among the group of patients 63 subjects were diagnosed with allergy and 65 subjects with asthma, respectively. DNA was isolated from peripheral blood lymphocytes of patients as well as controls and gene polymorphisms were analyzed by restriction fragments length polymorphism polymerase chain reaction (RFLP-PCR). We reported that the 140A/G LF (OR 4.78; 95% CI 3.07–7.24), the −33C/G OSF-2 OR 3.48; 95% CI 2.19–5.52) and the −33G/G OSF-2 (OR 16.45; 95% CI 6.71–40.30) genotypes were associated with an increased risk of chronic rhinosinusitis with nasal polyps among analyzed group of patients. Moreover, the group of patients without allergy or asthma indicated the association of the −33C/G (OR 3.72; 95% CI 2.24–6.19 and OR 15.11; 95% CI 5.91–38.6) and −33G/G (OR 3.73; 95% CI 2.24–6.19 and OR 14.07; 95% CI 5.47–36.16) genotypes of the OSF-2 as wells as 140A/G (OR 3.89; 95% CI 2.40–6.31 and OR 3.62; 95% CI 2.45–5.34) genotype of OSF-2 with an increased risk of chronic rhinosinusitis with nasal polyps. Finally, it was also found that the selected group of patients with allergy or asthma indicated a very strong association of the −33C/G (OR 2.40; 95% CI 1.23–4.69 and OR 2.40; 95% CI 1.23–4.69, respectively) and −33G/G (OR 16.01; 95% CI 5.77–44.41 and OR 17.90; 95% CI 6.53–49.05, respectively) genotypes of the OSF-2 as wells as 140A/G (OR 3.22; 95% CI 1.74–6.11 and OR 3.25; 95% CI 1.75–6.04, respectively) genotypes with an increased risk of chronic rhinosinusitis with nasal polyps. Thus, our results suggest that LF and OSF-2 gene polymorphisms may have deep impact on the risk of rhinosinusitis nasal polyps’ formation which may also depend on asthma or allergy. Our results showed that the 140A/G polymorphism of LF gene and the −33C/G polymorphism of the OSF-2 gene may be associated with the risk of chronic rhinosinusitis with nasal polyps in a Polish population.
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References
Becker DG (2003) Sinusitis. J Long Term Eff Med Implants 13(3):175–194
Thomas M, Yawn BP, Price D, Lund V, Mullol J, Fokkens W (2008) European position paper on rhinosinusitis, nasal polyps group. EPOS primary care guidelines: European position paper on the primary care diagnosis, management of rhinosinusitis, nasal polyps 2007: a summary. Prim Care Respir J 17(2):79–89
Vaidyanathan S, Barnes M, Williamson P, Hopkinson P, Donnan PT, Lipworth B (2011) Treatment of chronic rhinosinusitis with nasal polyposis with oral steroids followed by topical steroids: a randomized trial. Ann Intern Med 154(5):293–302
Ramakrishnan VR, Kennedy DW (2011) Advances in the surgical management of chronic sinusitis and nasal polyps. Curr Allergy Asthma Rep 11(3):220–229
Norlander T, Brönnegård M, Stierna P (1999) The relationship of nasal polyps, infection, and inflammation. Am J Rhinol 13(5):349–355
Pawankar R (2003) Nasal polyposis: an update: editorial review. Curr Opin Allergy Clin Immunol 3(1):1–6
Rinia AB, Kostamo K, Ebbens FA, van Drunen CM, Fokkens WJ (2007) Nasal polyposis: a cellular-based approach to answering questions. Allergy 62(4):348–358
Norris RA, Damon B, Mironov V, Kasyanov V, Ramamurthi A, Moreno-Rodriguez R, Trusk T, Potts JD, Goodwin RL, Davis J, Hoffman S, Wen X, Sugi Y, Kern CB, Mjaatvedt CH, Turner DK, Oka T, Conway SJ, Molkentin JD, Forgacs G, Markwald RR (2007) Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. J Cell Biochem 101(3):695–711
Liu Z, Kim J, Sypek JP, Wang IM, Horton H, Oppenheim FG, Bochner BS (2004) Gene expression profiles in human nasal polyp tissues studied by means of DNA microarray. J Allergy Clin Immunol 114(4):783–790
Nadolska B, Frączek M, Kręcicki T, Kocięba M, Zimecki M (2010) Lactoferrin inhibits the growth of nasal polyp fibroblasts. Pharmacol Rep 62(6):1139–1147
Zhang L, Han D, Wang Z (1998) Lysozyme and lactoferrin in human uncinate process mucosa during chronic sinusitis. Zhonghua Er Bi Yan Hou Ke Za Zhi 33:219–221
Psaltis AJ, Wormald PJ, Ha KR, Tan LW (2008) Reduced levels of lactoferrin in biofilm-associated chronic rhinosinusitis. Laryngoscope 118:895–901
Psaltis AJ, Bruhn MA, Ooi EH, Tan LW, Wormald PJ (2007) Nasal mucosa expression of lactoferrin in patients with chronic rhinosinusitis. Laryngoscope 117:2030–2035
Teplyuk NM, Haupt LM, Ling L, Dombrowski C, Mun FK, Nathan SS, Lian JB, Stein JL, Stein GS, Cool SM, van Wijnen AJ (2009) The osteogenic transcription factor Runx2 regulates components of the fibroblast growth factor/proteoglycan signaling axis in osteoblasts. J Cell Biochem 107(1):144–154
Jiang D, Liang J, Campanella GS, Guo R, Yu S, Xie T, Liu N, Jung Y, Homer R, Meltzer EB, Li Y, Tager AM, Goetinck PF, Luster AD, Noble PW (2010) Inhibition of pulmonary fibrosis in mice by CXCL10 requires glycosaminoglycan binding and syndecan-4. J Clin Invest 120(6):2049–2057
Pawankar R, Nonaka M (2007) Inflammatory mechanisms and remodeling in chronic rhinosinusitis and nasal polyps. Curr Allergy Asthma Rep 7(3):202–208
Hwang PM, Vogel HJ (1998) Structure-function relationships of antimicrobial peptides. Biochem Cell Biol 76:235–246
Rogan MP, Taggart CC, Greene CM et al (2004) Loss of microbicidal activity and increased formation of biofilm due to decreased lactoferrin activity in patients with cystic fibrosis. J Infect Dis 190:1245–1253
Fannon M, Forsten KE, Nugent MA (2000) Potentiation and inhibition of bFGF binding by heparin: a model for regulation of cellular response. Biochemistry 39:1434–1445
Velliyagounder K, Kaplan JB, Furgang D, Legarda D, Diamondd G, Parkin RE et al (2003) One of two human lactotransferrin variants exhibits increased antibacterial and transcriptional activation activities and is associated with localized juvenile periodontitis. Infect Immun 71(11):6141–6147
Kruzel ML, Bacsi A, Choudhury B, Sur S, Boldogh I (2006) Lactoferrin decreases pollen antigen-induced allergic airway inflammation in a murine model of asthma. Immunology 119:159–166
Bournazou I, Mackenzie KJ, Duffin R, Rossi AG, Gregory CD (2010) Inhibition of eosinophil migration by lactoferrin. Immunol Cell Biol 88:220–223
Artym J, Zimecki M, Kruzel M (2004) Normalization of peripheral blood cell composition by lactoferrin in cyclophosphamide-treated mice. Med Sci Monit 10:BR84–BR89
Stankovic KM, Goldsztein H, Reh DD, Platt MP, Metson R (2008) Gene expression profiling of nasal polyps associated with chronic sinusitis and aspirin-sensitive asthma. Laryngoscope 118(5):881–889
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This study was supported by Medical University of Lodz (503/7-124-04/503-01).
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Zielinska-Blizniewska, H., Sitarek, P., Milonski, J. et al. Association of the −33C/G OSF-2 and the 140A/G LF gene polymorphisms with the risk of chronic rhinosinusitis with nasal polyps in a Polish population. Mol Biol Rep 39, 5449–5457 (2012). https://doi.org/10.1007/s11033-011-1345-6
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DOI: https://doi.org/10.1007/s11033-011-1345-6