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PDIA3 mRNA expression and IL-2, IL-4, IL-6, and CRP levels of acute kidney allograft rejection in rat

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Abstract

Kidney transplantation to treat end-stage renal disease has evolved rapidly from the first successful transplantations to the current widespread use of grafts from both cadaveric and living donors. But acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate possible mechanisms, we describe a comparison between differentially proteins expression and immune markers profile (IL-2, IL-4, IL-6, and CRP) of acute rejection and the controls. Through quantitative real-time RT-PCR confirmation, PDIA3 mRNA and protein expression levels in serum and transplanted kidney in experiment group was significantly (P < 0.05) higher than that in control group. Immunity analysis showed that plasma IL-2, IL-4, IL-6, and CRP levels were higher in experimental rats than those in control rats. Our data thus indicate that PDIA3 might be potentially involve into the occurence and development of acute rejection response in renal transplantation and increased plasma IL-2, IL-4, IL-6, and CRP levels play an important role to prevent acute kidney allograft rejection in rats.

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Abbreviations

IL-2:

Interleukin-2

IL-4:

Interleukin-4

IL-6:

Interleukin-6

CRP:

C-reactive protein

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Acknowledgments

The work is supported by science fund from health bureau of Chongqing city (2010-2-042): effect of loss of balance of Th17/Treg cell subgroup on renal transplantation acute reject reaction and science fund from Chongqing Medical University (XBYB2008001): study of proteomics in rat allograft renal transplantation reject reaction.

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Authors and Affiliations

  1. Department of Urology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China

    Gang Chen, Ming Zhao Xiao & Yun Ri Fu

  2. Department of Emergency, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China

    Jie Mi

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  1. Gang Chen
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  2. Jie Mi
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  3. Ming Zhao Xiao
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  4. Yun Ri Fu
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Correspondence to Ming Zhao Xiao.

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Chen, G., Mi, J., Xiao, M.Z. et al. PDIA3 mRNA expression and IL-2, IL-4, IL-6, and CRP levels of acute kidney allograft rejection in rat. Mol Biol Rep 39, 5233–5238 (2012). https://doi.org/10.1007/s11033-011-1321-1

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  • DOI: https://doi.org/10.1007/s11033-011-1321-1

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