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Isolation of a gene encoding for a class III peroxidase in female flower of Corylus avellana L.

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Abstract

Hazelnut is a monoecious species characterized by mid-winter blooming and sporophytic incompatibility. The molecular mechanisms at the basis of the female flower development and of the pollen-stigma interaction are little known, although pollination in this species is a critical factor to ensure good yield. Differential display technique was used to study genes expressed during the female flower development, comparing styles before emergence from the bud and styles at full bloom. The full-length cDNA clone, designated CavPrx (Corylus avellana peroxidase) and isolated in mature styles, was characterized as a sequence encoding for a 330 amino acids protein, containing all the conserved features of class III peroxidases. CavPrx resulted expressed only in styles, with a peak in mature styles pollinated with compatible pollen. Class III peroxidases are expressed in several different plant tissue types and are involved in a broad spectrum of physiological processes. Until now, four peroxidases expressed in the stigma were identified in Arabidopsis thaliana and Senecio squalidus: they were assumed to be possibly involved in pollen–pistil interaction, pollen tube penetration/growth and/or in defence against pathogens. CavPrx is the first gene for a floral peroxidase isolated in hazelnut and its expression pattern suggests a possible role in the pollination process.

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Acknowledgments

The research was funded by Ministero dell’Istruzione, dell’Università e della Ricerca (MiUR) (projects “Pollen-stigma interaction and genetic determinants of sporophytic self-incompatibility in hazelnut (Corylus avellana L.)”—PRIN 2005; “Proteome and genetic expression analysis for the study of the factors responsible of sporophytic self-incompatibility in hazelnut”—PRIN 2007) and by Fondazione Cassa di Risparmio di Torino. Fondazione Cassa di Risparmio di Cuneo contributed funding a research grant.

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  1. Dipartimento di Colture Arboree, Università degli Studi di Torino, via Leonardo Da Vinci 44, 10095, Grugliasco, Turin, Italy

    Chiara Beltramo, Daniela Torello Marinoni, Irene Perrone & Roberto Botta

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  1. Chiara Beltramo
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  2. Daniela Torello Marinoni
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Correspondence to Daniela Torello Marinoni.

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Beltramo, C., Torello Marinoni, D., Perrone, I. et al. Isolation of a gene encoding for a class III peroxidase in female flower of Corylus avellana L.. Mol Biol Rep 39, 4997–5008 (2012). https://doi.org/10.1007/s11033-011-1296-y

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