Abstract
Infectious hypodermal and haematopoietic necrosis virus (IHHNV) has been detected widely in penaeid culture facilities in Asia and the Americas. IHHNV infection on sub-adult and postlarvae of the giant freshwater prawn, Macrobrachium rosenbergii which had caused up to 80% mortalities was first reported in Southeast Taiwan in 2006. In Malaysia, although, there has been no report on IHHNV infections in M. rosenbergii, preliminary work suggests that there is an urgent need to setup a screening protocol for IHHNV for both wild and cultured populations. In this study, polymerase chain reaction based screening was carried out on 30 randomly sampled berried wild M. rosenbergii before and after spawning. All samples did not showed any sign of IHHNV infection. However, the results showed that 20% of the samples were IHHNV positive. Sequence analysis of the amplified band using NCBI-BLAST showed that the putative IHHNV sequence had 98% nucleotide sequence (388 bp) identity with the IHHNV isolate AC-05-005 non-structural protein 1 gene and seven other IHHNV strains in the data bank further affirming the suggestion on the presence of IHHNV in wild freshwater prawn populations in Malaysia.
Similar content being viewed by others
References
Anderson IG, Law AT, Shariff M, Nash G (1990) A parvo-like virus in the giant freshwater prawn, Macrobrachium rosenbergii. J Invertebr Pathol 55:447–449
Arcier JM, Herman F, Lightner DV, Redman R, Mari J, Bonami JR (1999) A viral disease associated with mortalities in hatchery-reared postlarvae of the giant freshwater prawn Macrobrachium rosenbergii. Dis Aquat Org 38:177–181
Bell TA, Lightner DV (1984) IHHN virus: infectivity and pathogenicity studies in penaeus stylirostris and penaeus vannamei. Aquaculture 38:185–194
Bing Yang, Xiao-Ling Song, Jie Huang, Cheng-Yin Shi, Li Liu (2007) Evidence of existence of infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp cultured in China. Vet Microbiol 120:63–70
Bonami JR, Trumper B, Mari J, Brehelin M, Lightner DV (1990) Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps. J Gen Virol 71(Pt 11):2657–2664
Zhang Chong, Yuan Jun-fa, Shi Zheng-li (2007) Molecular epidemiological investigation of infectious hypodermal and hematopoietic necrosis virus and taura syndrome virus in Penaeus vannamei cultured in China. Virol Sin 22(5):380–388
Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1659
Flegel TW (2006) Detection of major penaeid shrimp viruses in Asia a historical perspective with emphasis on Thailand. Aquaculture 258:1–33
Hsia HL, Chen LL, Peng SE, Yu HT, Lo CF, Kou GH (2003) Comparison of genomic sequence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) between Taiwan and other geographical isolates. Fish Pathol 38(4):177–179
Hsieh CY, Chuang PC, Chen LC, Tu C, Chien MS, Huang HC, Kao HF, Tung MC, Tsai SS (2006) Infectious hypodermal and haematopoietic necrosis virus (IHHNV) infections in giant freshwater prawn, Macrobrachium rosenbergii. Aquaculture 258:73–79
Jeanmougin F, Thompson JD, Gouy M, Higgins DG, Gibson TJ (1998) Multiple sequence alignment with Clustal X. Trends Biochem Sci 23(10):403–405
Kim JH, Choresca CH Jr et al (2011) Detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Litopenaeus vannamei shrimp cultured in South Korea. Aquaculture 313(1–4):161–164
Lightner DV, Redman RM, Bell TA (1983) Infectious hypodermal and hematopoietic necrosis, a newly recognized virus disease of penaeid shrimp. J Invertebr Pathol 42:62–70
Lightner DV (1996) Epizootiology, distribution and the impact on international trade of two penaeid shrimp viruses in the Americas. Rev Sci Tech 15:576–601
Lightner DV (2003) The penaeid shrimp viral pandemics due to IHHNV, WSSV, TSV and YHV: history in the Americas and current status. In: Proceedings of the 32nd Joint UJNR Aquaculture Panel Symposium, Davis and Santa Barbara, California, USA, pp 17–20
Manual of diagnosis tests for aquatic animals (2003) Office international des epizooties (OIE), Paris. http://www.oie.int
Manual of diagnosis tests for aquatic animals (2009) Office international des epizooties (OIE), Paris. http://www.oie.int
Motte E, Yugcha E, Luzardo J, Castro F, Leclercq G, Rodríguez J, Miranda P, Borja O, Serrano J, Terreron M, Montalvo K, Narváez A, Tenorio N, Cedeño V, Mialhe E, Boulo V (2003) Prevention of IHHNV vertical transmission in the white shrimp Litopenaeus vannamei. Aquaculture 219:57–70
Nunan LM, Poulos BT, Lightner DV (2000) Use of polymerase chain reaction for the detection of infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp. Mar Biotechnol 2:39–328
Peng SE, Lo CF, Ho CH, Chang CF, Kou GH (1998) Detection of white spot baculovirus WSBV in giant freshwater prawn, Marcobrachium rosenbergii using polymerase chain reaction. Aquaculture 164:253–262
Rai P, Pradeep B, Safeena MP, Karunasagar I, Karunasagar I (2009) Simultaneous presence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) and the type A virus-related sequence in Penaeus monodon from India. Aquaculture 295:168–174
Rajendran KV, Vijayan KK, Santiago TC (1999) Experimental host range and histopathology of white spot syndrome virus (WSSV) in shrimps, prawns, crabs and lobsters from India with a note on the carrier/reservoir hosts. J Fish Dis 22:183–191
Sahul Hameed AS, Charles MX, Anilkumar M (2000) Tolerance of Macrobrachium rosenbergii to white spot syndrome virus. Aquaculture 183:207–213
Sahul Hameed AS, Yoganandhan K, Sri Widada J, Bonami JR (2004) Studies on the occurrence of Macrobrachium rosenbergii nodavirus and extra small virus-like particles associated with white tail disease of M. rosenbergii in India by PR-PCR detection. Aquaculture 238:127–133
Saksmerprome V, Puiprom O et al (2010) Detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) in farmed Australian Penaeus monodon by PCR analysis and DNA sequencing. Aquaculture 298(3–4):190–193
Shike H, Dhar AK, Burns JC, Shimizu C, Jousset FX, Klimpel KR, Bergoin M (2000) Infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito brevidensoviruses. Virology 277(1):167–177
Tamura K, Dudley J, Nei M & Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24(8): 1596–1599. doi:10.1093/molbev/msm092
Tang KFJ, Lightner DV (2002) Low sequence variation among isolates of infectious hypodermal and haematopoietic necrosis virus (IHHNV) originating from Hawaii and the Americas. Dis Aquat Org 49:93–97
Tang KFJ, Lightner DV (2006) Infectious hypodermal and hematopoietic necrosis virus (IHHNV)-related sequences in the genome of the black tiger prawn Penaeus monodon from Africa and Australia. Virus Res 118:185–191
Tang KFJ, Navarro SA, Lightner DV (2007) PCR assay for discriminating between infectious hypodermal and hematopoietic necrosis virus (IHHNV) and virus related sequence in the genome of Penaeus monodon. Dis Aquat Org 74:165–170
Tattersall P, Bergoin M, Booom ME, Brown KE, Linder RM, Muzyczka N, Parrish CR, Tijssen P (2005) Family Parvoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxanomy eight report of the international committee on taxonomy of viruses. Elsevier, Amsterdam, pp 353–369
Tung CW, Wang CS, Chen SN (1999) Histological and electron microscopic study on Macrobrachium muscle virus (MMV) infection in the giant freshwater prawn, Macrobrachium rosenbergii (de Man), cultured in Taiwan. J Fish Dis 22:1–5
Waikhom G, John KR, George MR, Jeyaseelan MJP (2006) Differential host passaging alters pathogenicity and induces genomic variation in white spot syndrome virus. Aquaculture 261:54–63
Yoganandhan K, Leartvibhas M, Sriwongpuk S, Limsuwan C (2006) White tail disease of the giant freshwater prawn Macrobrachium rosenbergii in Thailand. Dis Aquat Org 69(2–3):255–258
Acknowledgments
The authors are grateful to the staff of Pusat Penetasan Udang Galah (Prawn Hatchery Centre), Perak for their assistance rendered during sample collection and would like to express greatest appreciation to the fisheries officers, colleague, and staffs of National Fish Health Research Centre (NaFisH) for their encouragement and advice. This work was supported by Institute Agro-Biotech Malaysia (ABI), under grant No. 53-0203-1030 through sub-program and PPP grant PS166-2009A.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hazreen Nita, M.K., Kua, B.C., Bhassu, S. et al. Detection and genetic profiling of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infections in wild berried freshwater prawn, Macrobrachium rosenbergii collected for hatchery production. Mol Biol Rep 39, 3785–3790 (2012). https://doi.org/10.1007/s11033-011-1155-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-011-1155-x