Skip to main content
SpringerLink
Log in

Polymorphism of 5′ regulatory region of ovine FSHR gene and its association with litter size in Small Tail Han sheep

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Single nucleotide polymorphisms of 5′ regulatory region of follicle-stimulating hormone receptor (FSHR) gene were detected in two high prolificacy sheep breeds (Small Tail Han and Hu sheep) and two low prolificacy sheep breeds (Corriedale and Chinese Merino sheep) by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The results indicated that there were three genotypes (AA, AB and BB) detected by primer 1 in Hu sheep while only one genotype (AA) in other three sheep breeds, and frequencies of AA, AB and BB genotypes in Hu sheep were 0.700, 0.225 and 0.075, respectively. There were three genotypes (EE, EF and EG) detected by primer 3 in Small Tail Han sheep while only EE genotype occurred in other three sheep breeds, and frequencies of EE, EF and EG genotypes in Small Tail Han sheep were 0.775, 0.200 and 0.025, respectively. No polymorphism was detected in four sheep breeds by primer 2 and primer 4. The sequencing results showed that there were two nucleotide mutations (g. −681T>C and g. −629C>T) in genotype BB compared with AA for primer 1. As for primer 3, two mutations (g. −197G>A and g. −98T>C) in genotype EF compared with EE and two mutations (g. −200G>A and g. −197G>A) in genotype EG compared with EE. The heterozygous ewes with EG or EF had 0.89 (P < 0.05) or 0.42 (P < 0.05) lambs more than homozygous ewes (EE genotype) in Small Tail Han sheep, respectively, while there was no significant difference on litter size between EG and EF ewes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Gharib SD, Wierman ME, Shupnik MA, Chin WW (1990) Molecular biology of pituitary gonadotropins. Endocr Rev 11:177–199

    Article  PubMed  CAS  Google Scholar 

  2. Ranniki AS, Zhang FP, HuhtaniemiI T (1995) Ontogeny of follicle stimulating hormone receptor gene expression in the rat testis and ovary. Mol Cell Endocrinol 107:199–208

    Article  Google Scholar 

  3. Fan J, Deng XH (1998) Advance on follicle stimulating hormone. Reprod Contracept 18(2):122–125

    Google Scholar 

  4. Livshyts G, Podlesnaja S, Kravchenko S, Sudoma I, Livshits L (2009) A distribution of two SNPs in exon 10 of the FSHR gene among the women with a diminished ovarian reserve in Ukraine. J Assist Reprod Genet 26:29–34

    Article  PubMed  Google Scholar 

  5. Sprengel R, Braun T, Nikolics K, Segaloff DL, Seeburg PH (1990) The testicular receptor for follicle stimulating hormone: structure and functional expression of cloned cDNA. Mol Endocrinol 4:525–530

    Article  PubMed  CAS  Google Scholar 

  6. Abdennebi L, Monget P, Pisselet C, Remy JJ, Salesse R, Monniaux D (1999) Comparative expression of luteinizing hormone and follicle-stimulating hormone receptors in ovarian follicles from high and low prolific sheep breeds. Biol Reprod 60:845–854

    Article  PubMed  CAS  Google Scholar 

  7. Gu BH, Park JM, Baek KH (2010) Genetic variations of follicle stimulating hormone receptor are associated with polycystic ovary syndrome. Int J Mol Med 26:107–112

    PubMed  CAS  Google Scholar 

  8. Achrekar SK, Modi DN, Meherji PK, Patel ZM, Mahale SD (2010) Follicle stimulating hormone receptor gene variants in women with primary and secondary amenorrhea. J Assist Reprod Genet 27:317–326

    Article  PubMed  Google Scholar 

  9. Balkan M, Gedik A, Akkoc H, Izci Ay O, Erdal ME, Isi H, Budak T (2010) FSHR single nucleotide polymorphism frequencies in proven fathers and infertile men in Southeast Turkey. J Biomed Biotechnol. doi:10.1155/2010/640318

  10. Tu YR (1989) The sheep and goat breeds in China. Shanghai Science and Technology Press, Shanghai, pp 51, 57, 72, 80

  11. Song ML, Ma XM, Wang JM (2006) Single nucleotide polymorphism in goat FSHR gene and its association with superovulation. Acta Ecol Anim Domastici 27(4):25–28

    Google Scholar 

  12. Zhu J, Yang SL, Ouyang XX, Liu HX, Hu SG (2007) Relationship between polymorphism of follicle stimulating hormone receptor gene and litter size traits in goats. Grass Feed Livest (1):12–15

  13. Sairam MR, Subbarayan VSR (1997) Characterization of the 5′ flanking region and potential control elements of the ovine follitropin receptor gene. Mol Reprod Dev 48:480–487

    Article  PubMed  CAS  Google Scholar 

  14. Wei WC, Xu SZ, Xin HL (2001) Cloning and analysis of partial sequence of follicle-stimulating hormone receptor (FSHR) gene of Chinese Small Tail Han Sheep. Rev China Agric Sci Technol 3(6):61–65

    Google Scholar 

  15. Wei WC, Xu SZ (2002) Characterization and polymorphism of the 5′-flanking region of bovine follicle-stimulating hormone receptor (FSHR) gene. Acta Vet Zootech Sin 33:417–423

    Google Scholar 

  16. Yang WC, Li SJ, Tang KQ, Hua GH, Zhang CY, Yu JN, Han L, Yang LG (2010) Polymorphisms in the 5′ upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows. Anim Reprod Sci 119:172–177

    Article  PubMed  CAS  Google Scholar 

  17. Aittomäki K, Lucena JL, Pakarinen P, Sistonen P, Tapanainen J, Gromoll J, Kaskikari R, Sankila EM, Lehväslaiho H, Engel AR, Nieschlag E, Huhtaniemi I, de la Chapelle A (1995) Mutation in the follicle-stimulating hormone receptor gene causes hereditary hypergonadotropic ovarian failure. Cell 82:959–968

    Article  PubMed  Google Scholar 

  18. Zhang SJ, Xiong YZ, Deng CY, Zheng R, Jiang SW, Xiao SM, Xia Y, Xu JX, Liu XH, Wang CF, Yuan Z, Gong SY (2002) Study on follicle stimulating hormone receptor as candidate gene for litter size in pigs. J Huazhong Agric Univ 21:506–508

    CAS  Google Scholar 

  19. Lei XQ, Chen H, Yuan ZF, Lei CC, Xu TS, Sun WB (2002) Study on marker on bovine twinning trait with SNP of FSHR gene. Yunnan J Anim Sci Vet Med (4):28–29

Download references

Acknowledgments

This work was supported by National Key Technology Research and Development (R&D) Program of China (No. 2008BADB2B01, No. 2006BAD01A11 and No. 2006BAD13B08), by the earmarked fund for China Agriculture Research System (No. CARS-39), by National Key Basic R&D Program of China (No. 2006CB102105), by Beijing Natural Science Foundation of China (No. 5042017), and by Special Fund for Basic Scientific Research of Institute of Animal Science, Chinese Academy of Agricultural Sciences (ywf-td-1 and 2009td-1).

Author information

Authors and Affiliations

  1. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    M. X. Chu, D. W. Huang, T. Feng, G. L. Cao, L. Fang, R. Di, Q. Q. Tang, Y. H. Ma & K. Li

  2. Department of Physiology and Pathophysiology, Peking University Health Science Center and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, 100191, People’s Republic of China

    X. H. Guo

  3. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, People’s Republic of China

    C. J. Feng & Y. Li

Authors
  1. M. X. Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. X. H. Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. J. Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. W. Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. L. Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L. Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. R. Di
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Q. Q. Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Y. H. Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. K. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. X. Chu.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chu, M.X., Guo, X.H., Feng, C.J. et al. Polymorphism of 5′ regulatory region of ovine FSHR gene and its association with litter size in Small Tail Han sheep. Mol Biol Rep 39, 3721–3725 (2012). https://doi.org/10.1007/s11033-011-1147-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-011-1147-x

Keywords

Search

Navigation