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Induction of toll-like receptor 2 positive antigen-presenting cells in spleen of pristane-induced arthritis in rats

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Abstracts

Toll-like receptors (TLRs) have been found to contribute to the pathogenesis of rheumatoid arthritis (RA). The aim of this study is to investigate the regulation and potential role of TLR2 in spleen of pristane-induced arthritis (PIA) rat, which can be used to further understand the mechanisms of RA. Arthritis in DA rats was induced by pristane. TLR2 expression in spleen was detected by real-time quantitative PCR and western blotting, and TLR2 expression at both mRNA and protein levels was upregulated in PIA rats. Peptidoglycan (PGN) was systemically administrated to PIA rats, and arthritis severity was evaluated macroscopically and microscopically. Results showed that systemic administration of PGN to PIA rats obviously deteriorated arthritis severity. TLR2 expression on splenocytes and different types of immune cells was measured by flow cytometry. And it was found that TLR2 was mainly expressed on antigen-presenting cells (APCs) of spleen, and the proportion of TLR2+ dendritic cells and macrophages in spleen of PIA rats was increased remarkably. Thus, we conclude that the induction of TLR2+ APCs in spleen may participate in the maintenance of PIA.

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Acknowledgments

We should express gratitude to Mr. Fujun Zhang, Ms.Yan Han and Qilan Ning for their expertise and assistance in the experiments. The work was supported by grants from the National Natural Science Foundation of China (30801027, 30630058 and 81070030).

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Authors and Affiliations

  1. Department of Genetics and Molecular Biology, Xi’an Jiaotong University School of Medicine, Western Yanta Road 76, Xi’an, P.O. Box 81, 710061, Shaanxi, China

    Wenhua Zhu, Liesu Meng, Congshan Jiang, Weikun Hou, Jing Xu, Bo Wang & Shemin Lu

  2. Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi’an, 710061, Shaanxi, China

    Wenhua Zhu, Liesu Meng, Congshan Jiang, Weikun Hou, Jing Xu, Bo Wang & Shemin Lu

  3. Department of Epidemiology and Health Statistics, Xi’an Jiaotong University School of Medicine, Xi’an, 710061, Shaanxi, China

    Shemin Lu

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  1. Wenhua Zhu
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  2. Liesu Meng
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Correspondence to Liesu Meng.

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Zhu, W., Meng, L., Jiang, C. et al. Induction of toll-like receptor 2 positive antigen-presenting cells in spleen of pristane-induced arthritis in rats. Mol Biol Rep 39, 3667–3673 (2012). https://doi.org/10.1007/s11033-011-1141-3

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  • DOI: https://doi.org/10.1007/s11033-011-1141-3

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