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Molecular characterization, expression pattern, polymorphism and association analysis of bovine ADAMTSL3 gene

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Abstract

A disintegrin-like and metalloprotease domain with thrombospondin type I motifs-like 3 (ADAMTSL3) is an important candidate gene for body measurement traits through marker-assisted selection (MAS). The objective of this study was to analyze SNP of bovine ADAMTSL3 gene and its specific expression in tissues to explore its possible correlation with body measurement traits in Bos taurus. Our genomic structural analysis showed that bovine ADAMTSL3 shares much similarity with human ADAMTSL3. Gene expression analysis indicated that the order of specific tissue expression patterns of bovine ADAMTSL3 was the testis, heart, fat, stomach, small intestine, liver, lung, trachea, kidney, spleen, large intestine and muscle. Allele frequencies demonstrated significant variance in different local cattle breeds. Also, the T1532C SNP in exon 13 and C1899T SNP in exon 15 were significantly correlated with Body Length (BL), Chest Depth (CD), Heart Girth (HG) and Pin Bone Width (PBW). Furthermore, C1899T SNP marker had significant correlation with the PBW (P = 0. 0235) based on 867 individual analysis. In the total population, combination of T1532C and C1899T SNPs significantly affected on the BL (P = 0.0028), CD (P < 0.0001), HG (P = 0.0002) and PBW (P = 0.0049). These results suggest that the ADAMTSL3 gene, as one of target genes for body measurement traits in cattle breeds, could be used as a genetic marker to select excellent body type of cattle population in the animal breeding program.

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References

  1. Hurskainen TL, Hirohata S, Seldin MF, Apte SS (1999) ADAMTS5, ADAM-TS6, and ADAM-TS7, novel members of a new family of zinc metalloproteases. General features and genomic distribution of the ADAM-TSfamily. J Biolo Chem 274:25555–25563

    CAS  Google Scholar 

  2. Kuno K, Kanada N, Nakashima E, Fujiki F, Ichimura F, Matsushima K (1997) Molecular cloning of a gene encoding a new type of metalloproteinase-disintegrin family protein with thrombospondin motifs as an inflammation associated gene. Journal Biolo Chem 272:556–562

    CAS  Google Scholar 

  3. Apte SS (2004) A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motifs: the ADAMTS family. International J Biochem Cell Biology 36:981–985

    CAS  Google Scholar 

  4. Porter S, Clark IM, Kevorkian L, Edwards DR (2005) The ADAMTS metalloproteinases. Biochem J 386:15–27

    PubMed  CAS  Google Scholar 

  5. Huxley-Jones J, Apte SS, Robertson DL, Boot-Handford RP (2005) The characterisation of six ADAMTS proteases in the basal chordate Ciona intestinalis provides new insights into the vertebrate ADAMTS family. Int. J. Biochem. J Cell Biol 37:1838–1845

    CAS  Google Scholar 

  6. Hall NG, Klenotic P, Anand-Apte B, Apte SS (2003) ADAMTSL-3/punctin-2, a novel glycoprotein in extracellular matrix related to the ADAMTS family of metalloproteases. Matrix Biol 22:501–510

    PubMed  CAS  Google Scholar 

  7. Hirohata S, Wang LW, Miyagi M, Yan L, Seldin MF, Keene DR, Crabb JW, Apte SS (2002) Punctin, a novel ADAMTS-like molecule (ADAMTSL-1) in extracellular matrix. J Biolo Chem 277:12182–12189

    CAS  Google Scholar 

  8. Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1998) Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res 5:31–39

    PubMed  CAS  Google Scholar 

  9. Koo BH, Hurskainen T, Mielke K, Aung PP, Casey G, Autio-Harmainen H, Apte SS (2007) ADAMTSL3/punctin-2, a gene frequently mutated in colorectal tumors, is widely expressed in normal and malignant epithelial cells, vascular endothelial cells and other cell types, and its mRNA is reduced in colon cancer. Int J Cancer 121:1710–1716

    PubMed  CAS  Google Scholar 

  10. Gudbjartsson DF, Walters GB, Thorleifsson G, Stefansson H, Halldorsson BV, Zusmanovich P, Sulem P, Thorlacius S, Gylfason A, Steinberg S, Helgadottir A, Ingason A, Steinthorsdottir V, Olafsdottir EJ, Olafsdottir GH, Jonsson T, Borch-Johnsen K, Hansen T, Andersen G, Jorgensen T, Pedersen O, Aben KK, Witjes JA, Swinkels DW, den Heijer M, Franke B, Verbeek AL, Becker DM, Yanek LR, Becker LC, Tryggvadottir L, Rafnar T, Gulcher J, Kiemeney LA, Kong A, Thorsteinsdottir U, Stefansson K (2008) Many sequence variants affecting diversity of adult human height. Nat Genet 40:609–615

    PubMed  CAS  Google Scholar 

  11. He H, Liu X, Gu Y, Liu Y (2010) A novel 18-bp deletion mutation of the AMPD1 gene affects carcass traits in Qinchuan cattle. Mol Biol Rep 37(8):3945–3949

    PubMed  CAS  Google Scholar 

  12. Iglesias PP, Caffaro ME, Amadio AF, Arias Mañotti A, Poli MA (2010) CAPN1 markers in three Argentinean cattle breeds: report of a new InDel polymorphism within intron 17. Mol Biol Rep 38(3):1645–1649

    PubMed  Google Scholar 

  13. Juszczuk-Kubiak E, Flisikowski K, Wiciska K (2009) A new SNP in the 3’UTR region of the bovine calpain small subunit (CAPNS1) gene. Mol Biol Rep 37(1):473–476

    PubMed  Google Scholar 

  14. Gilbert RP, Bailey DRC, Shannon NH (1993) Linear body measurements of cattle before and after 20 years of selection for post weaning gain when fed two different diets. J Anim Sci 71:1712–1720

    PubMed  CAS  Google Scholar 

  15. Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt-chloroform extraction of DNA from blood and tissues. Trends Genet 5:391

    PubMed  CAS  Google Scholar 

  16. Wang H, Zhu ZM, Wang HL, Yang SL, Li K (2007) Molecular cloning, mapping, and expression analysis of the EIF4A2 gene in pig. Biochem Genetics 45:51–62

    CAS  Google Scholar 

  17. Huang HG, Zhao WM, Tang ZL, Yang SL, Wu ZF, Zhao SH, Cui WT, Mu YL, Chu MX, Li K (2009) Characterization of porcine MMP-2 and its association with immune traits. Gene 435:63–71

    PubMed  CAS  Google Scholar 

  18. Bundock PC, Cross K, Shapter FM, Henry RJ (2006) Robust allele-specific polymerase chain reaction markers developed for single nucleotide polymorphisms in expressed barley sequences. Theor Appl Genet 112:358–365

    PubMed  CAS  Google Scholar 

  19. Imyanitov EN, Buslov KG, Suspitsin EN, Kuligina ES, Belogubova EV, Grigoriev MY, Togo AV, Hanson KP (2002) Improved reliability of allele-specific PCR. Biotechniques 33:484–490

    PubMed  CAS  Google Scholar 

  20. Nasis O, Thompson S, Hong T, Sherwood M, Radcliffe S, Jackson L, Otevrel T (2004) Improvement in sensitivity of allele-specific PCR facilitates reliable noninvasive prenatal detection of cystic fibrosis. Clin Chem 50:694–701

    PubMed  CAS  Google Scholar 

  21. Nei M, Roychoudhury AK (1974) Sampling variance of heterozygosity and genetic distance. Genetics 76:379–390

    PubMed  CAS  Google Scholar 

  22. Nei M, Li WH (1979) Mathematic model for studying genetic variation in terms of restriction endonucleaes. PNAS 76:5269–5273

    PubMed  CAS  Google Scholar 

  23. Liu YF, Zan LS, Li K, Zhao SP, Xin YP, Lin Q, Tian WQ, Wang ZW (2010) A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds. Mol Biol Rep 37:429–434

    PubMed  CAS  Google Scholar 

  24. Liu Y, Zan L, Zhao S, Xin Y, Li L, Cui W, Tang Z, Li K (2010) Molecular characterization, polymorphism of bovine ZBTB38 gene and association with body measurement traits in native Chinese cattle breeds. Mol Biol Rep 37(8):4041–4049

    PubMed  CAS  Google Scholar 

  25. Shi YY, He L (2005) SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Res 15:97–98

    PubMed  CAS  Google Scholar 

  26. Ardlie KG, Kruglyak L, Seielstad M (2002) Patterns of linkage disequilibrium in the human genome. Nature Reviews Genetics 3:299–309

    PubMed  CAS  Google Scholar 

  27. Mateescu RG, Zhang Z, Tsai K, Phavaphutanon J, Burton-wurster NI, Quaas R, Murphy K, Acland GM, Todhunter RJ (2005) Analysis of allele fidelity, polymorphic information content, and density of microsatellites in a genome-wide screening for hip dysplasia in a crossbreed pedigree. J Heredity 96:847–853

    CAS  Google Scholar 

  28. Ren G, Chen H, Zhang LZ, Lan XY, Wei TB, Li MJ, Jing YJ, Lei CZ, Wang JQ (2010) A coding SNP of LHX4 gene is associated with body weight and body length in bovine. Mol Biol Rep 37(1):417–422

    PubMed  CAS  Google Scholar 

  29. Zhang B, Chen H, Guo Y, Zhang L, Zhao M, Lan X, Zhang C, Pan C, Hu S, Wang J, Lei C (2009) Associations of polymorphism within the GHSR gene with growth traits in Nanyang cattle. Mol Biol Rep 36(8):2259–2263

    PubMed  CAS  Google Scholar 

  30. Lettre G, Jackson AU, Gieger C, Schumacher FR, Berndt SI, Sanna S, Eyheramendy S, Voight BF, Butler JL, Guiducci C, Illig T, Hackett R, Heid IM, Jacobs KB, Lyssenko V, Uda M, Boehnke M, Chanock SJ, Groop LC, Hu FB, Isomaa B, Kraft P, Peltonen L, Salomaa V, Schlessinger D, Hunter DJ, Hayes RB, Abecasis GR, Wichmann HE, Mohlke KL, Hirschhorn JN (2008) Identification of ten loci associated with height highlights new biological pathways in human growth. Nat Genet 40:584–591

    PubMed  CAS  Google Scholar 

  31. Weedon MN, Lango H, Lindgren CM, Wallace C, Evans DM, Mangino M, Freathy RM, Perry JRB, Stevens S, Hall AS, Samani NJ, Shields B, Prokopenko I, Farrall M, Dominiczak A, Johnson T, Bergmann S, Beckmann JS, Vollenweider P, Waterworth DMV, Mooser CNA, Palmer AD, Morris WH, Ouwehand MC, Munroe PB, Hattersley AT, McCarthy MI, Frayling TM (2008) Genome-wide association analysis identifies 20 loci that influence adult height. Nat Genet 40:575–583

    PubMed  CAS  Google Scholar 

  32. Sovio U, Bennett AJ, Millwood IY, Molitor J, O’Reilly PF, Timpson NJ, Kaakinen M, Laitinen J, Haukka J, Pillas D, Tzoulaki I, Molitor J, Hoggart C, Coin LJ, Whittaker J, Pouta A, Hartikainen AL, Freimer NB, Widen E, Peltonen L, Elliott P, McCarthy MI, Jarvelin MR (2009) Genetic determinants of height growth assessed longitudinally from infancy to adulthood in the northern finland birth cohort 1966. PLoS Gene 5:e1000409

    Google Scholar 

  33. Soranzo N, Rivadeneira F, Chinappen-Horsley U, Malkina I, Richards JB, Hammond N, Stolk L, Nica A, Inouye M, Hofman A, Stephens J, Wheeler E, Arp P, Gwilliam R, Jhamai PM, Potter S, Chaney A, Ghori MJ, Ravindrarajah R, Ermakov S, Estrada K, Pols HA, Williams FM, McArdle WL, van Meurs JB, Loos RJ, Dermitzakis ET, Ahmadi KR, Hart DJ, Ouwehand WH, Wareham NJ, Barroso I, Sandhu MS, Strachan DP, Livshits G, Spector TD, Uitterlinden AG, Deloukas P (2009) Meta-analysis of genome-wide scans for human adult stature identifies novel loci and associations with measures of skeletal frame size. PLoS Gene 5:e1000445

    Google Scholar 

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Acknowledgments

The authors are grateful to Dr. Bin Wu for the critical reading and editing of the manuscript. This work was supported by the China National “863” Program (2008AA1010), the Changjiang Scholars and Innovative Research Team Support Plan of The Ministry of Education (IRt0940), GMO new varieties major project (2008ZX08007-002), the Fundamental Research Funds for the Central Universities (GK201002040) and National Key Scientific Program (2009CB941604). Also, the bovine populations were supported by excellent research team of livestock gene resource and breed improvement of Chinese Academy of Agricultural Sciences, Qinchuan Beef Cattle Breeding Center of Shaanxi Province, Nanyang, Jiaxian and Xianan Cattle Breeding Center of Henan Province, Luxi Cattle Breeding Center of Shandong Province, and Snow Dragon Beef Cattle Breeding Center of Liaoning Province (P.R. China).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, People’s Republic of China

    Yongfeng Liu, Linsen Zan, Shuanping Zhao, Yaping Xin & Yang Jiao

  2. Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Shuanping Zhao & Kui Li

  3. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, Shaanxi, 710062, People’s Republic of China

    Yongfeng Liu

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  1. Yongfeng Liu
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  2. Linsen Zan
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Correspondence to Linsen Zan or Kui Li.

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Liu, Y., Zan, L., Zhao, S. et al. Molecular characterization, expression pattern, polymorphism and association analysis of bovine ADAMTSL3 gene. Mol Biol Rep 39, 1551–1560 (2012). https://doi.org/10.1007/s11033-011-0894-z

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