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Mineral trioxide aggregate promotes odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp stem cells

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Abstract

Mitogen-activated protein kinase (MAPK) pathways are involved in stem cell differentiation. However, the odontoblastic differentiation-inducing effects by mineral trioxide aggregate (MTA) via MAPK pathways have not been clarified in human dental pulp stem cells (DPSCs). In this study we investigated the effects of MTA on cell viability and production of differentiation markers, and the involvement of MAPK signaling pathways in cultured human DPSCs. Cells were cultured with MTA, and the viability and differentiation productions of the cells were determined using the MTT assay and real-time PCR analysis, respectively. MAPK activation was measured by western blotting. MTA at concentrations of 20 and 10 mg/ml was toxic for human DPSCs. MTA significantly increased the expression of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), type I collagen (COLI), osteocalcin (OCN) and bone sialoprotein (BSP) mRNAs and induced the phosphorylation of p42 and p44 (p42/44), p38 and c-Jun N-terminal kinases 1 and 2 (JNK1/2) MAPK. Furthermore, the inhibitor of p42/44 MAPK attenuated the MTA-induced odontoblastic differentiation. These data indicated that MTA-induced odontoblastic differentiation of human DPSCs was via MAPK pathways, which may play a key role in the repair responses of dentin-pulp-like complexes.

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Acknowledgments

We are grateful to Prof. Wenli Yan for the linguistic revision of the manuscript. The work is supported by National Key Technologies R&D Program of the Eleventh-five Year Plan, the Ministry of Science and Technology of China (2007BAI18B01).

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Authors and Affiliations

  1. Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

    Xiu Zhao, Wenxi He, Zhongchun Tong, Shiting Li & Longxing Ni

  2. Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

    Zhi Song

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  1. Xiu Zhao
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  2. Wenxi He
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  4. Zhongchun Tong
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Correspondence to Longxing Ni.

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Zhao, X., He, W., Song, Z. et al. Mineral trioxide aggregate promotes odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp stem cells. Mol Biol Rep 39, 215–220 (2012). https://doi.org/10.1007/s11033-011-0728-z

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  • DOI: https://doi.org/10.1007/s11033-011-0728-z

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