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Influence of sCD40L on gastric cancer cell lines

Molecular Biology Reports volume 38, Article number: 5459 (2011) Cite this article

Abstract

The CD40 signaling pathway plays a key role in tumor cell proliferation, differentiation, and apoptosis. Gastric cancer usually possesses a higher level of CD40 expression than normal tissue. We evaluated inhibition of soluble CD40 ligand (sCD40L) in apoptosis induction of gastric cancer cells. Gastric cancer cells (AGS and BGC-823) were incubated with sCD40L. Cell viability and cell cycle were determined by methyl-tetrazolium (MTT) assay and flow cytometry, respectively. The results showed that sCD40L hindered cell growth, arrested cells at G0/G1 phase and induced apoptosis. In conclusion, sCD40L suppress growth of gastric cancer cells through apoptosis induction and cell cycle quiescence. This work will provided a new approach to gene therapy of gastric cancer.

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (No. 30872943), the Natural Science Foundation of Jiangsu Province (BK2010231) and the social development projects of Suzhou (No. SS0711) and postgraduate innovative plan project of Jiangsu Province in 2009 (No: CX09B-033Z).

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Affiliations

  1. Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Suzhou, 215006, China

    Rui Li, Wei-Chang Chen, Xue-Qin Pang & Wen-Yan Tian

  2. Institute of Medical Biotechnology, Soochow University, Suzhou, 215007, China

    Xue-Guang Zhang

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  1. Rui Li
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  2. Wei-Chang Chen
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  3. Xue-Qin Pang
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Correspondence to Wei-Chang Chen.

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Cite this article

Li, R., Chen, WC., Pang, XQ. et al. Influence of sCD40L on gastric cancer cell lines. Mol Biol Rep 38, 5459 (2011). https://doi.org/10.1007/s11033-011-0702-9

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  • DOI: https://doi.org/10.1007/s11033-011-0702-9

Keywords

  • Apoptosis
  • sCD40L
  • Cell cycle
  • Gastric cancer
  • AGS
  • BGC-823