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Molecular Biology Reports

, Volume 38, Issue 5, pp 3013–3016 | Cite as

Genetic diversity and statistical parameters of 15 autosomal STR loci in the Pomeranian Subpopulation of Espirito Santo State, Brazil

  • Beatriz Candida Silva
  • Eldamária de Vargas Wolfgramm
  • Vitor Resende da Costa Aguiar
  • Frederico Scott Varela Malta
  • Amanda Mafia de Castro
  • Alessandro Clayton de Souza Ferreira
  • Flavia de Paula
  • Iúri Drumond LouroEmail author
Article

Abstract

Allelic frequencies and other population data analysis are reported for the 15 autosomal Short Tandem Repeats (STR) loci included in the PowerPlex®16 kit (CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, Penta D, Penta E, TPOX, TH01 and vWA) in Pomeranian’s descendants from the Espirito Santo State (ES), Brazil, third largest population of Pomeranian’s descendants in the world. They chose the mountain region of the state for their preferred geographic location, and they have a very peculiar lifestyle with a selective mating behavior which has maintained their characteristics as a relatively pure subpopulation. Blood samples were obtained from 82 unrelated volunteers from 11 different cities of Espirito Santo State, where there are the Pomeranian’s descendants. All 15 loci analyzed showed Power of Discrimination (PD) values > 0.75. Except the TPOX locus, all analyzed loci were at Hardy–Weinberg equilibrium. This subpopulation has not yet been characterized for STR allelic frequencies used for forensic and genetic identification studies.

Keywords

STRs Allelic frequency PowerPlex®16 Pomeranian subpopulation Brazil 

Notes

Acknowledgments

We would like to thank Prodimol BiotecnologiaTM for kindly providing Promega kits. BC Silva was supported by a FAPES scholarship.

References

  1. 1.
    Domingues RB, Aquino CCH, Santos JG, Silva ALP, Kuster GW (2006) Prevalence and impact of headache and migraine among Pomeranians in Espirito Santo, Brazil. Arq Neuropsiquiatr 64:954–957PubMedCrossRefGoogle Scholar
  2. 2.
    Lee M, Park S, Kim D, Kim M, Ryu H, Cho Y (2009) Genetic variation of three autosomal STR Loci D21S1435, D21S1411, and D21S1412 in Korean population. Mol Biol Rep. doi  10.1007/s11033-009-9679-z
  3. 3.
    Miller AS, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA for human nucleated cells. Nucleic Acids Res 16:1215PubMedCrossRefGoogle Scholar
  4. 4.
    Excoffier L, Laval G, Schneider S (2005) Arlequin version 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedGoogle Scholar
  5. 5.
    Tereba A (1999) Tools for analysis of population statistics. Profile in DNA 14-16 (free software distributed by the authors at website http://www.promega.com/geneticidtools/)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Beatriz Candida Silva
    • 1
  • Eldamária de Vargas Wolfgramm
    • 1
  • Vitor Resende da Costa Aguiar
    • 1
  • Frederico Scott Varela Malta
    • 2
  • Amanda Mafia de Castro
    • 2
  • Alessandro Clayton de Souza Ferreira
    • 2
  • Flavia de Paula
    • 1
  • Iúri Drumond Louro
    • 1
    Email author
  1. 1.Núcleo de Genética Humana e Molecular Departamento de Ciências Biológicas, Centro de Ciências Humanas e NaturaisUniversidade Federal do Espírito SantoVitóriaBrazil
  2. 2.Departamento de Genética HumanaInstituto Hermes PardiniBelo HorizonteBrazil

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