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Molecular Biology Reports

, Volume 38, Issue 5, pp 3001–3011 | Cite as

α-Dystrobrevin distribution and association with other proteins in human promyelocytic NB4 cells treated for granulocytic differentiation

  • V. V. BorutinskaiteEmail author
  • K.-E. Magnusson
  • R. Navakauskiene
Article

Abstract

Dystrobrevins (DBs) bind directly to dystrophin and are prominent components of the dystrophin-associated protein complex (DAPC) that links the cytoskeleton to the extracellular matrix. They are involved in brain development, synapse formation and plasticity, as well as water and ion homeostasis. However, the role of DB in non-muscular cells is not clear. In this study, we show that different α-dystrobrevin isoforms are present in promyelocytic leukemia (NB4) cells. Only the biggest α-dystrobrevin isoform (DB-α), which can be important for its function, was expressed in the membrane fraction of NB4 cells; the other α-DB isoforms were found in the hydrophilic cell fractions. Employing the immunoprecipitation and mass spectrometry, we identified novel α-DB-interacting proteins involved in cytoskeleton reorganization (actin, tropomyosin, gelsolin, tubulin) and signal transduction process (stathmin, prohibitin, RIBA) during proliferation and differentiation of NB4 cells. Our results suggest that α-DB isoforms play a central role in cytoskeleton reorganization via their multiple interactions with actin and actin-associating proteins and may participate in signal transduction process during NB4 cell granulocytic differentiation via directly and non directly associated proteins.

Keywords

α-dystrobrevin Histone deacetylase inhibitor BML-210 Retinoic acid Granulocytic differentiation 

Abbreviations

α-DB

α-dystrobrevin

ATRA

All-trans retinoic acid

HDACI

Histone deacetylase inhibitor

Notes

Acknowledgements

This research was supported by the Swedish Institute (Visby Program No. 00879/2009), the Swedish Research Council, Lithuanian State Science and Studies Foundation (No.V-12/2009) and EU Marie Curie Programme Fellowship for V.V. Borutinskaite.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • V. V. Borutinskaite
    • 2
    • 1
    Email author
  • K.-E. Magnusson
    • 1
  • R. Navakauskiene
    • 2
  1. 1.Division of Medical Microbiology, Department of Clinical and Experimental MedicineLinköping UniversityLinkopingSweden
  2. 2.Department of Developmental BiologyInstitute of BiochemistryVilniusLithuania

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