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Impact of ERCC2 gene polymorphism on HIV-1 disease progression to AIDS among North Indian HIV patients

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Abstract

HIV/AIDS remains to be one of the killing diseases of mankind. Host genetic response is one of the factor which determine susceptibility to HIV and disease progression to AIDS. The aim of the present study was to evaluate the impact of ERCC2 Lyc 751 Gln (excision repair cross complementing rodent repair deficiency, complementation group 2) polymorphism on HIV-1 susceptibility and disease progression to AIDS, as this gene has been reported to intervene in degrading retroviral cDNA before it integrates with the host DNA. This case control study included 300 HIV seropositive cases and an equal number of HIV seronegative controls. DNA was isolated from the blood samples of study subjects and genotyping of ERCC2 was conducted by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. The Gln/Gln genotype showed a significant variation between cases and controls (P = 0.047, OR 1.71, 95% CI 1.00–2.93), indicating a possible role of susceptibility in reference to controls and disease progression when compared within cases.

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Acknowledgements

The authors of this paper would like to appreciate Dr. Ajay Wanchu’s clinical staff for the kind help and assistance they have provided during sample collection and data recording.

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Authors and Affiliations

  1. Department of Biotechnology, Panjab University, Chandigarh, Chandigarh, 16 00 14, India

    Ranbir Chander Sobti, Nega Berhane, Salih Abdul Mahdi, Rupinder Kler & Seyed Ali Hosseini

  2. Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India

    Vijish Kuttiat & Ajay Wanchu

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  1. Ranbir Chander Sobti
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  2. Nega Berhane
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  3. Salih Abdul Mahdi
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Correspondence to Ranbir Chander Sobti.

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Sobti, R.C., Berhane, N., Mahdi, S.A. et al. Impact of ERCC2 gene polymorphism on HIV-1 disease progression to AIDS among North Indian HIV patients. Mol Biol Rep 38, 2945–2952 (2011). https://doi.org/10.1007/s11033-010-9958-8

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  • DOI: https://doi.org/10.1007/s11033-010-9958-8

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