Associations between GSTM1 and OGG1 Ser326Cys polymorphisms and smoking on chromosomal damage and birth growth in mothers
- 125 Downloads
The presenting study was investigated the associations between individual susceptibility and cigarette smoke on maternal chromosomal damage and neonatal birth growth in smoking mothers since little known about genetic susceptibility to cigarette smoke in relation to adverse pregnancy outcome such as birth growth. Sixty-one pregnant women who completed a questionnaire at Ankara Education and Research Hospital, Department of Obstetrics and Gynecology have enrolled in this study. GSTM1 and OGG1 ser326Cys gene polymorphisms were analysed by RFLP-PCR (Restriction Fragment Length Polymorphism-Polymerase Chain Reaction) as possible genetic factors affecting susceptibility to such health effects of smoking and chromosomal damage was performed by chromosomal aberration assay (CAA) in maternal blood lymphocytes. Maternal self-reported history of pregnancy smoking was informed by questionnaire declaration. Our results showed that maternal smoking had significant effect on chromosomal damage, birth weight, and length. The frequencies of CA in smokers was significantly higher than that of the nonsmokers (3.46 ± 2.06 and 2.00 ± 1.3, P = 0.001). Birth weight and length in smokers were significantly higher that of nonsmokers (3,355 g and 49.57 cm, P = 0.001; 3,639 g and 50.79 cm, P = 0.002). On the other hand, there was a slightly increased in the frequencies of CA and reduction birth weight and length in GSTM1 null and length in OGG1 variant genotypes, those differences were not statistically significant (P > 0.05); likely due to small sample size. Larger sample size needs to reach significance.
KeywordsMaternal smoking Birth weight Birth length GSTM1 OGG1 Ser326Cys Chromosomal aberrations
Experiments carried out in the present study comply with the current laws of the country in which the experiments were performed. The authors would like to give special thanks to all participants. We wish also thank to Assoc. Prof. Kenan Özköse for statistical analysis.
- 21.Ellard GA, Johnstone FD, Prescott RJ, Ji-Xian PW, Jian-Hua M (1996) Smoking during pregnancy: the dose dependence of birthweight deficits. Int J Obstet Gynecol 103:806–813Google Scholar
- 32.Tsai HJ, Liu X, Mestan K, Yu Y, Zhang S, Fang Y, Pearson C, Ortiz K, Zuckerman B, Bauchner H, Cerda S, StubbleWeld PG, Xu X, Wang X (2008) Maternal cigarette smoking, metabolic gene polymorphisms, and preterm delivery: new insights on G£E interactions and pathogenic pathways. Hum Genet 123:359–369PubMedCrossRefGoogle Scholar