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Molecular Biology Reports

, Volume 38, Issue 5, pp 2887–2894 | Cite as

Osteogenic differentiation of human periosteal-derived cells in a three-dimensional collagen scaffold

  • Young-Mo Ryu
  • Young-Sool Hah
  • Bong-Wook Park
  • Deok Ryong Kim
  • Gu Seob Roh
  • Jong-Ryoul Kim
  • Uk-Kyu Kim
  • Gyu-Jin Rho
  • Geun-Ho Maeng
  • June-Ho ByunEmail author
Article

Abstract

This study examined the osteogenic differentiation of cultured human periosteal-derived cells grown in a three dimensional collagen-based scaffold. Periosteal explants with the appropriate dimensions were harvested from the mandible during surgical extraction of lower impacted third molar. Periosteal-derived cells were introduced into cell culture. After passage 3, the cells were divided into two groups and cultured for 28 days. In one group, the cells were cultured in two-dimensional culture dishes with osteogenic inductive medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. In the other group, the cells were seeded onto a three-dimensional collagen scaffold and cultured under the same conditions. We examined the bioactivity of alkaline phosphatase (ALP), the RT-PCR analysis for ALP and osteocalcin, and measurements of the calcium content in the periosteal-derived cells of two groups. Periosteal-derived cells were successfully differentiated into osteoblasts in the collagen-based scaffold. The ALP activity in the periosteal-derived cells was appreciably higher in the three-dimensional collagen scaffolds than in the two-dimensional culture dishes. The levels of ALP and osteocalcin mRNA in the periosteal-derived cells was also higher in the three-dimensional collagen scaffolds than in the two-dimensional culture dishes. The calcium level in the periosteal-derived cells seeded onto three-dimensional collagen scaffolds showed a 5.92-fold increase on day 7, 3.28-fold increase on day 14, 4.15-fold increase on day 21, and 2.91-fold increase on day 28, respectively, compared with that observed in two-dimensional culture dishes. These results suggest that periosteal-derived cells have good osteogenic capacity in a three-dimensional collagen scaffold, which provides a suitable environment for the osteoblastic differentiation of these cells.

Keywords

Periosteal-derived cells Collagen scaffold Osteogenic differentiation 

Notes

Acknowledgements

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2007-331-E00237).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Young-Mo Ryu
    • 1
  • Young-Sool Hah
    • 2
  • Bong-Wook Park
    • 1
  • Deok Ryong Kim
    • 3
  • Gu Seob Roh
    • 4
  • Jong-Ryoul Kim
    • 5
  • Uk-Kyu Kim
    • 5
  • Gyu-Jin Rho
    • 6
  • Geun-Ho Maeng
    • 6
  • June-Ho Byun
    • 1
    Email author
  1. 1.Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Biomedical Center (BK21)Gyeongsang National University School of MedicineJinjuRepublic of Korea
  2. 2.Clinical Research Institutue of Gyeongsang National University HospitalJinjuRepublic of Korea
  3. 3.Department of Biochemistry, Institute of Health Sciences, Biomedical Center (BK21)Gyeongsang National University School of MedicineJinjuRepublic of Korea
  4. 4.Department of Anatomy, Institute of Health Sciences, Biomedical Center (BK21)Gyeongsang National University School of MedicineJinjuRepublic of Korea
  5. 5.Department of Oral and Maxillofacial Surgery, School of DentistryPusan National UniversityBusanRepublic of Korea
  6. 6.College of Veterinary MedicineGyeongsang National UniversityJinjuRepublic of Korea

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