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Evaluation of binding and thermodynamic characteristics of interactions between a citrus flavonoid hesperitin with protein and effects of metal ions on binding

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Abstract

The mechanism of interaction of a non-glycosidic citrus flavonoid, hesperitin (HES) with bovine serum albumin (BSA) was studied by UV–vis absorption, fluorescence, FT-IR, circular dichroism, fluorescence anisotropy and synchronous fluorescence spectroscopy in phosphate buffer of pH 7.4. Fluorescence data revealed that the fluorescence quenching of BSA by HES was the result of the formed complex of HES–BSA. The binding constants and thermodynamic parameters at four different temperatures, the location of binding, and the nature of binding force were determined. The hydrogen bonds interactions were found to be the predominant intermolecular forces to stabilize the complex. The conformation of BSA was discussed by synchronous fluorescence and CD methods. The alterations of protein secondary structure upon complexation with HES were evident from the gradual decrease in α-helicity. The distance between the donor (BSA) and acceptor (flavonoid) was calculated from the fluorescence resonance energy transfer and found to be 1.978 nm. Common ions viz., Zn2+, K+, Cu2+, Ni2+, Mn2+ and Co2+ were found to influence the binding of flavonoid to protein.

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Acknowledgments

We are grateful to the Council of Scientific and Industrial Research, New Delhi, for financial assistance (No. 01(2279)/08/EMR-II dated 20-11-2008). One of the authors (AHH) thanks the UGC, New Delhi for awarding the Fellowship for meritorious students in Science. Thanks are also due to the authorities of the Karnatak University, Dharwad, for providing the necessary facilities.

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Authors and Affiliations

  1. Department of Chemistry, Karnatak University, Dharwad, 580003, Karnataka, India

    Ashwini H. Hegde, B. Sandhya & J. Seetharamappa

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  1. Ashwini H. Hegde
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  2. B. Sandhya
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  3. J. Seetharamappa
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Correspondence to J. Seetharamappa.

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Hegde, A.H., Sandhya, B. & Seetharamappa, J. Evaluation of binding and thermodynamic characteristics of interactions between a citrus flavonoid hesperitin with protein and effects of metal ions on binding. Mol Biol Rep 38, 4921–4929 (2011). https://doi.org/10.1007/s11033-010-0634-9

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  • DOI: https://doi.org/10.1007/s11033-010-0634-9

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