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An easy method to detect the kinetics of CD44 antibody and its receptors on B16 cells using atomic force microscopy

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Abstract

CD44 is the principle cell surface receptor for the extracellular matrix. The altered expression or dysfunction of CD44 proteins contributes to numerous pathological processes. Therefore, it is very necessary to detect the distribution and density of CD44 proteins on cell surface. In this paper, the unbinding force between the tip of an atomic force microscope modified with anti-human CD44 antibody (a kind of CD44 pathway ligation proteins, currently used to induce the apoptosis of some types of tumors) and B16 (human melanoma cell line) cells was measured. The results indicated that the distribution of CD44 was nonuniform and represented clusters on B16 cell surface. And, the data of kinetics of CD44 antibody-antigen binding experiments indicated that the CD44 signal pathway in B16 cells could be blocked by anti-CD44 monoclonal antibody. This methodology can be extended to the evaluation and screening of molecular targeted drugs for pharmacological use.

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Acknowledgments

We express our thanks to Prof. Guanqun Yang (The First Affiliated Hospital, Jinan University, China) and Zhihong Liang (Analytical and Testing Center, Jinan University, China) for their help and hot discussion. This work was funded by the grants from China’s Guangzhou National Science Foundation (021190, 2003Z3-D2041) and National Natural Science Foundation of China (973 program projects, 2010CB833603).

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Authors and Affiliations

  1. Department of Chemistry and Institute for Nano-Chemistry, Jinan University, 510632, Guangzhou, China

    Hua Jin, Lina Ma, Hongyan Ye & Jiye Cai

  2. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, 510090, Guangzhou, China

    Hongxia Zhao

  3. The First Affiliated Hospital, Jinan University, 510632, Guangzhou, China

    Xianxian Chen

  4. Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Science, 215125, Suzhou, China

    Xun Huang

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  1. Hua Jin
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  2. Hongxia Zhao
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  3. Xianxian Chen
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  4. Lina Ma
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  5. Xun Huang
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  6. Hongyan Ye
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  7. Jiye Cai
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Correspondence to Jiye Cai.

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Jin, H., Zhao, H., Chen, X. et al. An easy method to detect the kinetics of CD44 antibody and its receptors on B16 cells using atomic force microscopy. Mol Biol Rep 38, 4495–4500 (2011). https://doi.org/10.1007/s11033-010-0580-6

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  • DOI: https://doi.org/10.1007/s11033-010-0580-6

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