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Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats

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Abstract

Two pairs of primers (P1 and P2) were designed to detect single nucleotide polymorphisms of exon 2 and intron 2 of bone morphogenetic protein 4 (BMP4) gene in both high fecundity breed (Jining Grey goat) and low fecundity breeds (Boer, Angora and Inner Mongolia Cashmere goats) by single strand conformation polymorphism. Results showed that no polymorphism was detected for exon 2 (primer P1) of BMP4 gene in four goat breeds. For intron 2 (primer P2), three genotypes (AA, AB and BB) were detected in Jining Grey and Inner Mongolia Cashmere goats, two genotypes (AB and BB) in Angora goats, and only one genotype (AA) in Boer goats. Sequencing revealed one mutation (2203G>A) of BMP4 gene in the genotype BB in comparison to the genotype AA. The differences of litter size between AA, AB and BB genotypes were not significant (P > 0.05) in Jining Grey goats. A pair of primer (P3) was designed to detect polymorphism in the 3′ flanking region of BMP4 gene that contained dinucleotide repeated sequence (CA) in the four goat breeds by microsatellite analysis. For primer P3, three genotypes (CC, CD and DD) were detected in four goat breeds. Sequencing revealed one more CA dinucleotide in genotype DD than in genotype CC. The Jining Grey does with genotype CC had 0.55 (P < 0.05) or 0.72 (P < 0.05) kids more than those with genotype CD or DD. These results preliminarily indicated that allele C of BMP4 gene is a potential DNA marker for improving litter size in goats.

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Acknowledgments

This work was supported by National High Technology Research and Development Program of China (2006AA10Z139), National Natural Science Foundation of China (30871773), National Key Basic Research and Development Program of China (2006CB102105), the earmarked fund for Modern Agro-industry Technology Research System of China (nycytx-39), Beijing Natural Sciences Foundation of China (6062023), and Special Fund for Basic Scientific Research of Institute of Animal Science, Chinese Academy of Agricultural Sciences (ywf-td-1 and 2009td-1).

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Authors and Affiliations

  1. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    M. X. Chu, T. Feng, R. Di, G. L. Cao, L. Fang, Y. H. Ma & K. Li

  2. Bioengineering College, Chongqing University, Chongqing, 400030, People’s Republic of China

    L. Lu & P. Q. Wang

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  1. M. X. Chu
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  2. L. Lu
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  3. T. Feng
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  4. R. Di
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  5. G. L. Cao
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  7. L. Fang
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  8. Y. H. Ma
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  9. K. Li
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Correspondence to M. X. Chu.

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Chu, M.X., Lu, L., Feng, T. et al. Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats. Mol Biol Rep 38, 4315–4320 (2011). https://doi.org/10.1007/s11033-010-0556-6

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  • DOI: https://doi.org/10.1007/s11033-010-0556-6

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