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Role of microRNA-21 and effect on PTEN in Kazakh’s esophageal squamous cell carcinoma

Molecular Biology Reports volume 38, pages 3253–3260 (2011)Cite this article

Abstract

The aim of this study was investigate the role of microRNA-21 (miR-21) and its regulation on phosphatase and tensin homolog deleted from chromosome-10 (PTEN) in Kazakh’s esophageal squamous cell carcinoma (ESCC). MiR-21 expressions were investigated in esophageal cancer cell line Eca109, and 18 pairs of Kazakh’s ESCC and adjacent normal tissues by real-time quantitative PCR (qRT-PCR). To evaluate the role of miR-21 and PTEN, cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells. Moreover, the expressions of PTEN were performed by Western blotting. In Eca109, when transfected with miR-21 mimics, accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40%, which resulted in the promotion of cell proliferation. However, when transfected with miR-21 inhibitor, expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79%, which resulted in the suppression of cell proliferation. Both of them had no effect on the level of PTEN mRNA. Compared with adjacent normal tissues, miR-21 expression was significantly higher in tumor (P < 0.05). Specifically, patients with cancer cell invasion deep into esophageal serosa showed significantly higher expression of miR-21. Protein expression of PTEN was significantly lower in tumor compared with normal tissues (P < 0.05); however, mRNA expression of PTEN had no obvious significance between them. Furthermore, there was a significantly inverse correlation between miR-21 expression and PTEN protein levels (p < 0.05). The author concluded that MiR-21 was overexpressed in vitro and ESCC, and promoted the cell proliferation, might target PTEN at post-transcriptional level, and regulated the cancer invasion in Kazakh’s ESCC.

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Acknowledgment

The project was supported by the National Natural Science Foundation (No. 30760223, 30860097) and the First Affiliated of Xinjiang Medical University Grant (No. 2008-YFY-01).

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Affiliations

  1. Medical Research Center, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, 830054, Xinjiang Uygur Autonomous Region, People’s Republic of China

    Wen-jing Ma, Guo-dong Lv, Aerziguli Tuersun, Qing Liu, Hui Liu, Shu-tao Zheng, Cong-gai Huang, Jun-guo Feng, Xing Wang, Ren-yong Lin & Xiao-mei Lu

  2. Department of Thoracic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi, 830054, Xinjiang Uygur Autonomous Region, People’s Republic of China

    Ilyar Sheyhidin

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  1. Wen-jing Ma
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  2. Guo-dong Lv
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  4. Qing Liu
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  5. Hui Liu
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  7. Cong-gai Huang
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  9. Xing Wang
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  10. Ren-yong Lin
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  11. Ilyar Sheyhidin
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  12. Xiao-mei Lu
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Correspondence to Xiao-mei Lu.

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Ma, Wj., Lv, Gd., Tuersun, A. et al. Role of microRNA-21 and effect on PTEN in Kazakh’s esophageal squamous cell carcinoma. Mol Biol Rep 38, 3253–3260 (2011). https://doi.org/10.1007/s11033-010-0480-9

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  • DOI: https://doi.org/10.1007/s11033-010-0480-9

Keywords

  • ESCC
  • MicroRNA-21
  • PTEN
  • Invasion
  • Kazakh