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Cloning and expression of stearoyl-CoA desaturase 1 (SCD-1) in the liver of the Sichuan white goose and landes goose responding to overfeeding

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Abstract

The EST sequence of goose (Anser cygnoides) Stearoyl-CoA desaturase 1(SCD-1) was obtained from a subtractive cDNA library. To further investigate the role of SCD-1 in lipid metabolism in geese, 5′-RACE and 3′-RACE were carried out in this study to obtain the complete cDNA sequence of goose SCD-1, which contained a 29-bp 5′ UTR, a 1074-bp open reading frame (ORF) encoding 357 amino acids, and a 125-bp 3′ UTR. The expression of SCD-1 was measured in several tissues, and the effects of overfeeding on the expression of SCD-1 were studied. The results of real time RT-PCR demonstrated that, compared to the brain, goose SCD-1 mRNA was more abundant in the liver. Overfeeding markedly increased the mRNA expression of SCD-1 in the liver of Sichuan White and Landes geese, and gene expression was markedly higher in the Sichuan White goose than in the landes goose. The mRNA abundance of SCD-1 in the liver had significant positive correlations with triacylglycerol (TG) content in liver lipids and in the levels of plasma insulin and very low-density lipoproteins (VLDL) levels in Sichuan white geese. However, the mRNA abundance of SCD-1 in the livers of Landes geese had only significant positive correlations with the TG content in liver lipids. In conclusion, SCD-1 is not only critical for hepatic steatosis in geese but is also important for the difference in lipid deposition in the livers of the two breeds.

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Acknowledgments

The work was supported by the National Waterfowl Industrial Technology System (No. nycytx-45-05).

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Authors and Affiliations

  1. Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan, 625014, People’s Republic of China

    Zhi-xiong Pan, Chun-chun Han, Ji-wen Wang, Liang Li, Hui Tang, Jia Lv & Feng Xu

  2. Institute of Animal Science and Veterinary Medicine, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang, 310021, People’s Republic of China

    Lizhi Lu

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  1. Zhi-xiong Pan
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  2. Chun-chun Han
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Correspondence to Ji-wen Wang.

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Pan, Zx., Han, Cc., Wang, Jw. et al. Cloning and expression of stearoyl-CoA desaturase 1 (SCD-1) in the liver of the Sichuan white goose and landes goose responding to overfeeding. Mol Biol Rep 38, 3417–3425 (2011). https://doi.org/10.1007/s11033-010-0451-1

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  • DOI: https://doi.org/10.1007/s11033-010-0451-1

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