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Characterization of a F-box gene up-regulated by phytohormones and upon biotic and abiotic stresses in grapevine

Abstract

F-box proteins are key components of the ubiquitin (Ub)/26S proteasome pathway that mediates selective degradation of regulatory proteins involved in a wide variety of cellular processes affecting eukaryotic cells. In plants, F-box genes form one of the largest multigene superfamilies and control many important biological functions. Among the F-box genes characterized to date only few have been involved in the regulation of plant defense responses. Moreover, no F-box genes have been studied and characterized in grapevine. Using a differential display approach we isolated a F-box gene (BIG-24.1), which is up-regulated during Botrytis cinerea infection of grapevine leaves. BIG-24.1 encodes a polypeptide of 386 amino acids with a conserved F-box domain in the N-terminus region and a kelch domain. By investigating expression profiles of BIG-24.1, we show that the gene expression is strongly stimulated in B. cinerea infected berries and in grapevine cells challenged by MAMP rhamnolipids, a non-host bacterium and an endophytic rhizobacterium. The gene is also strongly induced by abiotic stresses including UV-C and wounding or by salicylic acid, methyl-jasmonate, ethylene and abscisic acid that are known to be involved in defense signalling pathways. In addition, sequence analysis of the BIG-24.1 promoter revealed the presence of several regulatory elements involved in the activation of plant defense responses.

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Acknowledgments

We thank Cathy Hachet and Fanja Rabenoelina for technical support. This work was supported by a fund from Europôl’Agro.

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Correspondence to Stéphan Dorey.

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Sandra Paquis and Florence Mazeyrat-Gourbeyre have contributed equally to this work.

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Paquis, S., Mazeyrat-Gourbeyre, F., Fernandez, O. et al. Characterization of a F-box gene up-regulated by phytohormones and upon biotic and abiotic stresses in grapevine. Mol Biol Rep 38, 3327–3337 (2011). https://doi.org/10.1007/s11033-010-0438-y

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Keywords

  • Abiotic stress
  • Biotic stress
  • Defense responses
  • F-box
  • Phytohormones
  • Plant