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A novel human derived cell-penetrating peptide in drug delivery

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Abstract

Cell-penetrating peptides can carry a variety of biologically active molecules into cells. Here we have identified a novel CPP derived from the C-terminus of human extracellular superoxide dismutase (hC-SOD3) which was shown to be located throughout in the cytoplasm and nucleus by fluorescence microscopy investigation. Furthermore, when apoptin fused to hC-SOD3, it was translocated efficiently into HeLa cells resulting in antitumor activities. This study shows that hC-SOD3 has the potential to penetrate and translocate cargo molecules into cells and has no cytotoxicity at effective concentration.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Mailbox 365, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Jian Zhao, Peng Gao, Wei Xiao, Li-Qiang Fan, Su-Xia Li & Jian-Wen Liu

  2. Zhejiang Reachall Pharmaceutical Co. Ltd, Zhejiang, 322100, China

    Fu-Jun Wang

Authors
  1. Jian Zhao
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  2. Peng Gao
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  3. Wei Xiao
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  4. Li-Qiang Fan
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  5. Fu-Jun Wang
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  6. Su-Xia Li
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  7. Jian-Wen Liu
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Correspondence to Jian Zhao.

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Zhao, J., Gao, P., Xiao, W. et al. A novel human derived cell-penetrating peptide in drug delivery. Mol Biol Rep 38, 2649–2656 (2011). https://doi.org/10.1007/s11033-010-0406-6

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  • DOI: https://doi.org/10.1007/s11033-010-0406-6

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