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Molecular cloning, sequence identification and tissue expression profile of three novel genes Sfxn1, Snai2 and Cno from Black-boned sheep (Ovis aries)

Molecular Biology Reports volume 38, pages 1883–1887 (2011)Cite this article

Abstract

The complete coding sequences of three of Black-boned sheep (Ovis aries) genes Sfxn1, Snai2 and Cno were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) according to the conserved sequence information of the cattle or other mammals and known highly homologous sheep ESTs. Black-boned sheep Sfxn1 gene encodes a protein of 322 amino acids which has high homology with the Sfxn1 proteins of five species—cattle 98%, pig 95%, human 95%, rat 93%, and mouse 93%. Black-boned sheep Snai2 gene encodes a protein of 268 amino acids that has high identity with the Snai2 proteins of six species—cattle 99%, pig 94%, human 93%, dog 93%, rat 91%, and mouse 90%. Black-boned sheep Cno gene encodes a protein of 214 amino acids that has high homology with the Cno proteins of four species—cattle 97%, human 75%, mouse 67%, and rat 65%. The phylogenetic tree analysis demonstrated that Black-boned sheep Sfxn1, Snai2 and Cno proteins have close relationship with cattle Sfxn1, Snai2 and Cno proteins. The tissue expression analysis indicated that Black-boned sheep Sfxn1, Snai2 and Cno genes were expressed in a range of tissues including leg muscle, kidney, skin, longissimus dorsi muscle, spleen, heart and liver. Our experiment is the first to provide the primary foundation for further insight into these three sheep genes.

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Acknowledgments

This work was supported by the Natural Science Foundation Key Project of Yunnan Province (2006C0005Z), the National Nature Science Foundation of China (30760177 and 30860042) and the “863” Key Program (2008AA101001).

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Affiliations

  1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, People’s Republic of China

    Dongmei Xi, Yiduo He, Yongke Sun, Xiao Gou, Shuli Yang, Huaming Mao & Weidong Deng

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  1. Dongmei Xi
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  2. Yiduo He
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  3. Yongke Sun
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  4. Xiao Gou
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  5. Shuli Yang
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  7. Weidong Deng
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Correspondence to Weidong Deng.

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Dongmei Xi and Yiduo He have equal contribution to this paper.

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Xi, D., He, Y., Sun, Y. et al. Molecular cloning, sequence identification and tissue expression profile of three novel genes Sfxn1, Snai2 and Cno from Black-boned sheep (Ovis aries). Mol Biol Rep 38, 1883–1887 (2011). https://doi.org/10.1007/s11033-010-0306-9

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Keywords

  • Black-boned sheep
  • Sfxn1, Snai2 and Cno
  • Tissue expression analysis