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Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells

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Abstract

Fbxw8 is the F-box component of a SCF-like E3 ubiquitin ligase complex. Mice lacking Fbxw8 exhibit pathological defects in placenta and embryo similar to fetal growth retardation, suggesting a role of Fbxw8 in placentation. Proliferative capacity of trophoblast cells is very important in placental development. In this context, we revealed that Fbxw8 was expressed in four different human trophoblast cell lines. Silencing of Fbxw8 expression by siRNA inhibited the growth of choriocarcinoma JEG-3 cells. By Western blotting, cell cycle analysis, we showed that down-regulation of Fbxw8 by RNAi induced cell-growth arrest at G2/M phase through decreasing the levels of CDK1, CDK2, cyclin A and cyclin B1 and up-regulation of p27 at protein level. Conversely, over-expression of Fbxw8 led to the opposite effect. These results suggest that Fbxw8 plays an essential role in the proliferation of human trophoblast cells, especially JEG-3 cells, via G2/M phase transition in association with regulation of CDK1, CDK2, cyclin A, cyclin B1 and p27 expression.

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Acknowledgments

This study was supported by a grant of CAS Knowledge Innovation Program (KSCX2-YW-R-080) to EK Duan, grants from National Natural Science Foundation of China (no. 30971088 and 30811120431) to Wang HM, and Natural Science Foundation of Heilongjiang Province, China (QC2009C47) to Lin P.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Harbin Medical University, Harbin, 150081, China

    Ping Lin, Binjiahui Zhao, Feng Lin, Haifeng Zou, Leiyu Liu & Xiaoguang Yu

  2. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1, Bei Chen West Road, Chaoyang District, Beijing, 100101, China

    Jiejun Fu, Cheng Zhu & Hongmei Wang

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  1. Ping Lin
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  2. Jiejun Fu
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  9. Xiaoguang Yu
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Correspondence to Hongmei Wang or Xiaoguang Yu.

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Ping Lin, Jiejun Fu contributed equally to this work.

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Lin, P., Fu, J., Zhao, B. et al. Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells. Mol Biol Rep 38, 1741–1747 (2011). https://doi.org/10.1007/s11033-010-0288-7

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  • DOI: https://doi.org/10.1007/s11033-010-0288-7

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