Abstract
A cDNA clone designated arf1 was isolated from a physic nut (Jatropha curcas L.) endosperm cDNA library which encodes a small GTP-binding protein and has significant homology to ADP-ribosylation factors (ARF) in plants, animals and microbes. The cDNA contains an open reading frame that encodes a polypeptide of 181 amino acids with a calculated molecular mass of 20.7 kDa. The deduced amino acid sequence showed high homology to known ARFs from other organisms. The products of the arf1 obtained by overexpression in E. coli revealed the specific binding activity toward GTP. The expression of arf1 was observed in flowers, roots, stems and leaves as analyzed by RT-PCR, and its transcriptional level was highest in flowers. In particular, the accumulation of arf1 transcripts was different under various environmental stresses in seedlings. The results suggest that arf1 plays distinct physiological roles in Jatropha curcas cells.
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Acknowledgments
This research is supported by “11th Five-Year Plan” to support science and technology project (NO.2006BAD07A04), Ministry of Science and Technology international cooperation project (NO.2006DFB63400) and National Natural Science Foundation project on the surface (NO.30670204). We also thank the China Scholarship Council for their support.
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Xiaobo Qin and Fanrong Lin contributed equally to this work.
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Fig. 1
Alignment of the deduced amino acid sequences of ARF proteins from various sources. The amino acid sequences from various biological sources were aligned by CLUSTALX, and their similarity was compared and analyzed by DNAMAN. Three conserved structures (P, G’, and G) for GTP-binding were indicated with the black lines. A potential myristoylation site was indicated with an arrow head. Abbreviations of the represented species and GeneBank accession numbers were PtARF1: Populus tomentosa (AAR18698), DcARF002: Daucus carota (ABB03801), AtARF2: Arabidopsis thaliana (Q9SRC3), HsARF1: Homo sapiens (NP_001019399), GhARF1: Gossypium hirsutum (CAD12855), ScARF3: Saccharomyces cerevisiae (CAA99291), OsARF: Oryza sativa (AAT77289), ZmARF: Zea mays (CAA56351), MmARF: Medicago sativa (AAR29293), StARF1: Solanum tuberosum (ABB16972) (JPEG 914 kb)
Fig. 2
Phylogenetic tree analysis of small GTP-binding proteins from various biological sources. Phylogenetic tree for the amino acid sequences of ARFs and from various biological sources were constructed by MEGA. The percentage bootstrap values calculated from 1000 replications are indicated around the internal nodes. Abbreviations of the corresponding proteins involved in species and their GeneBank accession numbers: EgARF, Elaeis guineensis (ACF06579); OsARF, Oryza sativa (AAT77289); DcARF002, Daucus carota (ABB03801); MtARF1, Medicago truncatula (CAI29265); MsARF, Medicago sativa (AAR29293.1); StARF1, Solanum tuberosum (ABB16972); GmARF, Glycine max (AAD17207); GhARF1, Gossypium hirsutum (CAD12855); PtARF1, Populus tomentosa (AAR18698); PtARF2, Populus tremuloides (AAO63780); ZmARF, Zea mays (CAA56351); MmARF, Mus musculus (BAA13494); MmARF2, Mus musculus (BAA13491); BtARF5, Bos taurus (AAI34696); CaARF, Candida albicans (AAB23053.2); ScARF1, Saccharomyces cerevisiae (CAA98769.1); ScARF3, Saccharomyces cerevisiae (CAA99291.1); RnARF6, Rattus norvegicus (NP_077066); HsARF1, Homo sapiens (NP_001019399); HsARF3, Homo sapiens (NP_001650); HsARF4, Homo sapiens (NP_001651); HsARF5, Homo sapiens (NP_001653); HsARF6, Homo sapiens (NP_001654); AtARF1, Arabidopsis thaliana (M95166.1); AtARF2, Arabidopsis thaliana (Q9SRC3); AtARF3, Arabidopsis thaliana (X77385); AtRab2, Arabidopsis thaliana (CAA70498); AtRab11, Arabidopsis thaliana (CAA70112); AtRab18, Arabidopsis thaliana (CAA48178); AtRop1, Arabidopsis thaliana (AAC78390); AtRop2, Arabidopsis thaliana (AAC78391); AtRop4, Arabidopsis thaliana (AAC78242); AtRop6, Arabidopsis thaliana (AAC78241) (JPEG 1538 kb)
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Qin, X., Lin, F., Lii, Y. et al. Molecular analysis of ARF1 expression profiles during development of physic nut (Jatropha curcas L.). Mol Biol Rep 38, 1681–1686 (2011). https://doi.org/10.1007/s11033-010-0280-2
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DOI: https://doi.org/10.1007/s11033-010-0280-2