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New SNP of the porcine Perilipin 2 (PLIN2) gene, association with carcass traits and expression analysis in skeletal muscle

Molecular Biology Reports volume 38, pages 1575–1583 (2011)Cite this article

Abstract

PLIN2 (perilipin 2) is a cytosolic protein that promotes the formation and stabilization of the intracellular lipid droplets, organelles involved in the storage of lipid depots. Porcine PLIN2 gene represents a biological and positional candidate for fat deposition, a polygenic trait that affects carcass and meat quality. The aim of the present study was to screen PLIN2 gene for polymorphisms, to evaluate the association with carcass quality traits, and to investigate the gene expression in skeletal muscle. Six new single nucleotide polymorphisms (SNP) were detected by sequencing 32 samples from five pig breeds (Italian Large White, Italian Duroc, Italian Landrace, Belgian Landrace, Pietrain). Two SNP localized in introns, two in the 3′-untranslated region (UTR), and two missense SNP were found in exons. A 3′-UTR mutation (GU461317:g.98G>A), genotyped in 290 Italian Duroc pigs by High Resolution Melting, resulted significantly associated (P < 0.01) with average daily gain, feed conversion ratio, lean cuts and hams weight estimated breeding values. PLIN2 gene expression analysis in skeletal muscle of Italian Large White and Italian Duroc pigs divergent for backfat thickness and visible intermuscular fat showed a trend of higher expression level in pigs with higher intermuscular fat. These results suggest that PLIN2 can be a marker for carcass quality in pigs. Further investigation at both gene and protein level could elucidate its role on fat deposition.

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Acknowledgments

This work funded by the Italian Ministry for Education, University and Research under the Relevant National Interest Projects Programme 2006. The authors thank Dr. Emilio Scotti for the helpful technical assistance.

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Authors and Affiliations

  1. DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Via F.lli Rosselli 107, 42100, Reggio, Emilia, Italy

    Roberta Davoli, Greta Gandolfi, Silvia Braglia, Marco Comella, Paolo Zambonelli & Vincenzo Russo

  2. Associazione Nazionale Allevatori Suini (ANAS), Via Lazzaro Spallanzani 4/6, 00161, Rome, Italy

    Luca Buttazzoni

Authors
  1. Roberta Davoli
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  2. Greta Gandolfi
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  3. Silvia Braglia
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  4. Marco Comella
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  7. Vincenzo Russo
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Correspondence to Roberta Davoli.

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Davoli, R., Gandolfi, G., Braglia, S. et al. New SNP of the porcine Perilipin 2 (PLIN2) gene, association with carcass traits and expression analysis in skeletal muscle. Mol Biol Rep 38, 1575–1583 (2011). https://doi.org/10.1007/s11033-010-0266-0

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  • DOI: https://doi.org/10.1007/s11033-010-0266-0

Keywords

  • PLIN2
  • SNP
  • Gene expression
  • Pig breeds
  • Carcass quality
  • Fat deposition