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Relationship between surfactant protein A polymorphisms and allergic rhinitis in a Chinese Han population

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Abstract

The development of allergic rhinitis is considered to be determined by the interaction between genetic and environmental factors. Surfactant protein A (SP-A), a member of the collectin family of proteins, plays an important role in immune regulation. The purpose of this study was to investigate the association between SP-A polymorphisms and allergic rhinitis. We conducted a case–control association study on a Chinese Han population, comprising 216 adult individuals with AR and 84 healthy controls. A total of 9 single-nucleotide polymorphisms (SNPs) mapped to the SP-A were genotyped using PCR-based molecular identification methods. The frequency of A allele at amino acid 223 in the patient group was significantly higher than that in the control group after correcting for multiple testing (P = 0.006). The 1A2 allele haplotype in SFTPA2 was associated with decreased risk for allergic rhinitis, after applying Bonferroni corrections (P = 0.003). However, genetic variants of the SFTPA1 genes were not found to be associated with AR. In addition, no significant associations were established between any of the 9 SFTPA gene polymorphisms and the skin-prick test responses (P > 0.05). Further, no association was established between the 9 SNP loci and the levels of total serum immunoglobulin E (IgE) (P > 0.05). These results indicate that the gene polymorphism at the residue 223 in the carbohydrate recognition domain of SFTPA2 may be a genetic marker for the development of AR in the adult Chinese Han population.

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Abbreviations

SP-A:

Surfactant protein A

SNPs:

Single-nucleotide polymorphisms

IgE:

Total serum immunoglobulin E

AR:

Allergic rhinitis

cRFLP:

Converted restriction fragment length polymorphism

HWE:

Hardy–Weinberg equilibrium

LD:

Linkage disequilibrium

D:

Disequilibrium value

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Acknowledgment

This work was supported by the national key technology R&D program (Grant number 2007BA18B15).

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Authors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China

    Yuqin Deng, Shiming Chen, Jinhui Chen, Zezhang Tao, Yonggang Kong, Yu Xu, Bokui Xiao & Qing He

  2. Central Research Laboratory, Renmin Hospital of Wuhan University, Wuhan, China

    Qing He

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  1. Yuqin Deng
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  2. Shiming Chen
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  3. Jinhui Chen
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  4. Zezhang Tao
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  6. Yu Xu
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  7. Bokui Xiao
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  8. Qing He
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Corresponding author

Correspondence to Zezhang Tao.

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Yuqin Deng and Shiming Chen contributed equally to this study.

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Deng, Y., Chen, S., Chen, J. et al. Relationship between surfactant protein A polymorphisms and allergic rhinitis in a Chinese Han population. Mol Biol Rep 38, 1475–1482 (2011). https://doi.org/10.1007/s11033-010-0254-4

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  • DOI: https://doi.org/10.1007/s11033-010-0254-4

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