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Downregulation of ornithine decarboxylase by pcDNA-ODCr inhibits gastric cancer cell growth in vitro

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Abstract

Ornithine decarboxylase (ODC), the first rate-limiting enzyme of polyamine biosynthesis, was found to be associated with cell growth, proliferation and transformation. ODC gene expression in gastric cancer was increased and its level was positively correlated with the degree of malignity of gastric mucosa and development of gastric lesions. In order to evaluate the therapeutic effects of antisense RNA of ODC on gastric cancer, an antisense RNA of ODC expressing plasmid pcDNA-ODCr which delivered a 120 bp fragment complementary to the initiation codon of ODC gene was constructed and transfected to gastric cancer cells SGC7901 and MGC803. Expression of ODC in gastric cancer cells was determined by western blot. Cell proliferation was assessed by MTS assay. Cell cycle was analyzed by flow cytometry and Matrigel assay was performed to assess the ability of gastric cancer cell invasiveness. The results showed that the ODC gene expression in gastric cancer cells transfected with the pcDNA-ODCr was downregulated efficiently. Tumor cell proliferation was suppressed significantly, and cell cycle was arrested at G1 phase. Gastric cancer cells had reduced invasiveness after gene transfer. Our study suggested that antisense RNA of ODC expressing plasmid pcDNA-ODCr had antitumor activity by inhibiting the expression of ODC, and downregulation of ODC expression using a gene therapy approach might be a novel therapeutic strategy for gastric cancer.

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Acknowledgments

This work was supported by the Foundation of the Department of Science and Technology of Shandong Province (No: 2008GG30002030).

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Authors and Affiliations

  1. Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, 44#, Wenhua Xi Road, Jinan, Shandong, 250012, China

    Chun-xiao Xu, Yun-fei Yan, Ya-pei Yang, Bin Liu, Jia-xuan Xin, Shi-min Chen, Wei Wang & Xian-xi Liu

  2. Department of Colo-Proctology, The Affiliated Hospital to Shandong University of Tradition Chinese Medicine, Jinan, Shandong, 250011, China

    Chun-ying Jiang

  3. Clinical College, Shandong University of Tradition Chinese Medicine, Jinan, Shandong, 250014, China

    Zhao-xin Lu

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  1. Chun-xiao Xu
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Correspondence to Xian-xi Liu.

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Chun-xiao Xu, Yun-fei Yan, and Ya-pei Yang have contributed equally to this work.

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Xu, Cx., Yan, Yf., Yang, Yp. et al. Downregulation of ornithine decarboxylase by pcDNA-ODCr inhibits gastric cancer cell growth in vitro. Mol Biol Rep 38, 949–955 (2011). https://doi.org/10.1007/s11033-010-0188-x

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