Abstract
Channa marulius (Hamilton, 1822) is a commercially important freshwater fish and a potential candidate species for aquaculture. The present study evaluated partial Cytochrome b gene sequence of mtDNA for determining the genetic variation in wild populations of C. marulius. Genomic DNA extracted from C. marulius samples (n = 23) belonging to 3 distant rivers; Mahanadi, Teesta and Yamuna was analyzed. Sequencing of 307 bp Cytochrome b mtDNA fragment revealed the presence of 5 haplotypes with haplotype diversity value of 0.763 and nucleotide diversity value of 0.0128. Single population specific haplotype was observed in Mahanadi and Yamuna samples and 3 haplotypes in Teesta samples. The analysis of data demonstrated the suitability of partial Cytochrome b sequence in determining the genetic diversity in C. marulius population.
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The authors thank Sh. A.K. Pathak (Scientist and OIC ARIS Cell, NBFGR) for providing the locality map. Excellent technical cooperation from Sh. Akhilesh Mishra, Sh. Rajesh Kumar and Sh. R.S Sah is duly acknowledged.
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Habib, M., Lakra, W.S., Mohindra, V. et al. Evaluation of cytochrome b mtDNA sequences in genetic diversity studies of Channa marulius (Channidae: Perciformes). Mol Biol Rep 38, 841–846 (2011). https://doi.org/10.1007/s11033-010-0175-2
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DOI: https://doi.org/10.1007/s11033-010-0175-2