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Real-time reverse transcription–PCR expression profiling of porcine troponin I family in three different types of muscles during development

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Abstract

In this study, the expression profiling of three troponin I isoforms (TNNI1, TNNI2 and TNNI3) was investigated in two pig breeds differing in muscularity (Yorkshire and Meishan) at six stages (fetal 60 days and postnatal 3, 35, 60, 120, and 180 days) and three types of muscles (longissimus dorsi muscle, LD; semitendinosus, ST; cardiac muscle, CM) using relative real-time quantitative PCR. Significant differences of troponin I expression in three muscles were found between Yorkshire and Meishan breeds at some stages. The expression peak of TNNI1 and TNNI2 in LD and ST was at postnatal 35 or 60 days in Yorkshire and at postnatal 120 or 180 days in Meishan pigs, while it occurred in CM at postnatal 3 days in two pig breeds. The relative expression values of TNNI1 and TNNI2 were significantly higher in LD than ST at most of stages after birth. The expression ratio of TNNI2 versus TNNI1 favoured TNNI2 expression in ST and LD, but on the contrary in CM. The expression peak of TNNI3 occurred at postnatal 60 and 120 days in Yorkshire and Meishan pigs, respectively. TNNI1 and TNNI3 were co-expressed in CM during the fetal and earlier stages after birth.

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Acknowledgements

This study was supported financially by the Agricultural Innovation Fund of Hubei Province, the National High Technology Development Project (“863” project), National Natural Science Foundation of P. R. China (30500358), National Key Technology R&D Program during the 11th Five Year Plan (2008BADB2B02), the creative team project of Education Ministry of China (IRT0831) and the National “973’’ Program of P. R. China (2006CB102102).

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Authors and Affiliations

  1. Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    H. Yang, Z. Y. Xu, M. G. Lei, F. E. Li, C. Y. Deng, Y. Z. Xiong & B. Zuo

  2. Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    H. Yang, Z. Y. Xu, M. G. Lei, F. E. Li, C. Y. Deng, Y. Z. Xiong & B. Zuo

  3. Livestock and Poultry Breeding Center of Hubei Province, Wuhan, 430070, People’s Republic of China

    H. Yang

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  1. H. Yang
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  2. Z. Y. Xu
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  3. M. G. Lei
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  4. F. E. Li
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  5. C. Y. Deng
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  6. Y. Z. Xiong
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  7. B. Zuo
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Correspondence to B. Zuo.

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H. Yang and Z. Y. Xu contribute equally to this paper.

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Yang, H., Xu, Z.Y., Lei, M.G. et al. Real-time reverse transcription–PCR expression profiling of porcine troponin I family in three different types of muscles during development. Mol Biol Rep 38, 827–832 (2011). https://doi.org/10.1007/s11033-010-0172-5

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  • DOI: https://doi.org/10.1007/s11033-010-0172-5

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