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Analyses of functional IL10 and TNF-α genotypes in Behçet’s syndrome

Molecular Biology Reports volume 37, pages 3637–3641 (2010)Cite this article

Abstract

We aim to ascertain the possible involvement of functional IL10 and TNF-α promoter polymorphisms on the susceptibility to Behçet’s syndrome (BS), to examine whether IL10 and TNF-α genotypes might work synergistically influencing susceptibility to BS. IL10 −1082G/A, −819C/T and −592C/A and TNF −308G/A polymorphisms were analyzed in 102 Turkish patients with BS and 102 healthy subjects by using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). We have found no significant associations between IL10 −1082G/A, −819C/T, −592C/A, TNF-α −308G/A polymorphisms and BS. Also, no significant correlation was found between IL10 GCC, ACC, ATA haplotypes, GCC+/GCC+, GCC+/GCC−, GCC−/GCC− genotypes. There was no significant association between combined TNF-α/IL10 genotypes and BS. Our study indicates that functional TNF-α, IL10 genotypes or combined TNF-α, IL10 genotypes do not play a role in BS susceptibility in Turkish BS patients.

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Acknowledgments

This work was supported by Research Fund of Istanbul University. Project numbers: 412/13092005.

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Affiliations

  1. Department of Molecular Biology and Genetics, Science Faculty, Istanbul University, Istanbul, Turkey

    Omer Ateş, Levent Dalyan & Aysegul Topal-Sarıkaya

  2. Rheumatology Department, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey

    Gulen Hatemi & Vedat Hamuryudan

  3. Department of Medical Biology, Medical Faculty, Gaziosmanpasa University, 60100, Tokat, Turkey

    Omer Ateş

Authors
  1. Omer Ateş
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  2. Levent Dalyan
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  3. Gulen Hatemi
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  4. Vedat Hamuryudan
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  5. Aysegul Topal-Sarıkaya
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Correspondence to Omer Ateş.

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Ateş, O., Dalyan, L., Hatemi, G. et al. Analyses of functional IL10 and TNF-α genotypes in Behçet’s syndrome. Mol Biol Rep 37, 3637–3641 (2010). https://doi.org/10.1007/s11033-010-0015-4

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  • DOI: https://doi.org/10.1007/s11033-010-0015-4

Keywords

  • Behçet’s syndrome
  • TNF-α
  • IL10
  • Polymorphism