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Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue

Molecular Biology Reports volume 37, pages 3567–3574 (2010)Cite this article

Abstract

MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.

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Acknowledgments

We thank Jun Mo Kim and Jong-Gun Kim for assistance in collecting porcine tissue samples. This work was supported by a grant (20070301034036) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, 136-713, South Korea

    Ik Sang Cho, Jung Kim, Hye Youn Seo, Do Hwan Lim, Jae Sang Hong, Young Hee Park, Dae Cheul Park, Ki-Chang Hong, Kwang Youn Whang & Young Sik Lee

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  1. Ik Sang Cho
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Correspondence to Young Sik Lee.

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Ik Sang Cho, Jung Kim contributed equally to this work.

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Cho, I.S., Kim, J., Seo, H.Y. et al. Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue. Mol Biol Rep 37, 3567–3574 (2010). https://doi.org/10.1007/s11033-010-0005-6

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  • DOI: https://doi.org/10.1007/s11033-010-0005-6

Keywords

  • MicroRNA
  • Skeletal muscle
  • Adipose tissue
  • Pig