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A PCR-based SNP marker for specific authentication of Korean ginseng (panax ginseng) cultivar “Chunpoong”

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Abstract

Korean ginseng (Panax ginseng) has been developed as a horticultural crop due to the increasing demand in the world market. “Chunpoong” is an economically important cultivar with superior quality and high yield among nine cultivars of Korean ginseng. The aim of this work was to develop a simple technique for specific authentication of Chunpoong using DNA method. Molecular authentication of Chunpoong was investigated using DNA sequences of mitochondrial cytochrome oxidase subunit 2 (cox2) intron I and intron II regions. A single nucleotide polymorphism (SNP) specific to Chunpoong was detected and amplification refractory mutation system (ARMS)-PCR method was applied to specific identification of Chunpoong based on the SNP site. Ginseng samples collected from other locations were used to validate the SNP marker and the established method was determined to be effective. Thus, this work provides a rapid and reliable method for the specific identification of Chunpoong cultivar.

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Acknowledgements

This study was supported by KGCMVP for Technology Development Program of Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

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Authors and Affiliations

  1. Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, Gyeonggi-do, 446-701, Republic of Korea

    Hongtao Wang, Hua Sun, Woo-Saeng Kwon & Deok-Chun Yang

  2. Institute of Food Science and Technology, Yantai University, Yantai, China

    Hongtao Wang & Haizhu Jin

Authors
  1. Hongtao Wang
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  2. Hua Sun
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  3. Woo-Saeng Kwon
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  4. Haizhu Jin
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  5. Deok-Chun Yang
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Corresponding author

Correspondence to Deok-Chun Yang.

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Wang, H., Sun, H., Kwon, WS. et al. A PCR-based SNP marker for specific authentication of Korean ginseng (panax ginseng) cultivar “Chunpoong”. Mol Biol Rep 37, 1053–1057 (2010). https://doi.org/10.1007/s11033-009-9827-5

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  • DOI: https://doi.org/10.1007/s11033-009-9827-5

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