Abstract
The aim of this study to determine whether serum p53 protein and antibodies are associated with malignant tumors. A case–control study was conduct in 569 patients with various types of malignant tumors and 879 healthy controls. Serum p53 protein and antibodies were analyzed by enzyme-linked immunosorbent assay (ELISA).The rate of positive p53 protein in patients with various malignant tumors was 4.22% compared with 0.34% in healthy controls (P < 0.001). The rate of anti-p53 antibodies in patients with various malignant tumors was 14.59% compared with 1.02% in healthy controls (P < 0.001). The adjusted odd ratio (OR) for p53 protein was 17.55 (95% CI = 4.98–61.94). The adjusted odd ratio for anti-p53 antibodies was 14.27 (95% CI = 6.75–30.16). The study strongly suggested that serum p53 protein and antibody are associated with increased cancer risk and can be used as early serological markers in the diagnosis of malignancies tumors.
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References
Levine AJ, Momand J, Finlay CA (1991) The p53 tumour suppressor gene. Nature 351(6326):453–456
Charuruks N, Tangkijvanich P, Voravud N, Chatsantikul R, Theamboonlers A, Poovorawan Y (2001) Clinical significance of p53 antigen and anti-p53 antibodies in the sera of hepatocellular carcinoma patients. J Gastroenterol 36(12):830–836
Lenner P, Wiklund F, Emdin SO, Arnerlöv C, Eklund C, Hallmans G et al (1999) Serum antibodies against p53 in relation to cancer risk and prognosis in breast cancer: a population-based epidemiological study. Br J Cancer 79(5–6):927–932
Schneider J, Persek P, Braun A, Bauer P, Konietzko N, Wiesner B et al (1999) p53 protein, EGF receptor, and anti-p53 antibodies in serum from patients with occupationally derived lung cancer. Br J Cancer 80(12):1987–1994
Gumus E, Erdamar S, Demirel G, Horasanli K, Kendirci M, Miroglu C (2004) Association of positive serum anti-p53 antibodies with poor prognosis in kidney cancer patients. Int J Urol 11(12):1070–1077
Wu M, Chen Q, Cu X (2006) Testing serum p53 protein by sandwich ELISA. Chin J Clin Lab Sci 24(11):448 Chinese
Wu M, Chen Q, Wang Y (2003) Preparation of egg yolk immunoglobulin (IgY) against p53 protein expressed in E coli. J First Mil Med Univ 23(5):491–493 Chinese
Wu M, Chen Q, Cu X, Wang Y (2006) Development of ELISA method for detecting serum p53 antibodies. South China J Prev Med 32(6):19–21 Chinese
Shen H, Chen Q (2000) New concepts and methods of standardizing predictive value, accuracy and incorrect diagnostic rate. Anal Quant Cytol Histol 22(1):76–79
Balogh GA, Mailo DA, Corte MM, Roncoroni P, Nardi H, Vincent E et al (2006) Mutant p53 protein in serum could be used as a molecular marker in human breast cancer. Int J Oncol 28(4):995–1002
Mocci F, Nettuno M (2006) Plasma mutant-p53 protein and anti-p53 antibody as a marker: an experience in vinyl chloride workers in Italy. J Occup Environ Med 48(2):158–164
Müller M, Meyer M, Schilling T, Ulsperger E, Lehnert T, Zentgraf H et al (2006) Testing for anti-p53 antibodies increases the diagnostic sensitivity of conventional tumor markers. Int J Oncol 29(4):973–980
Shimada H, Ochiai T, Nomura F, Japan p53 Antibody Research Group (2003) Titration of serum p53 antibodies in 1, 085 patients with various types of malignant tumors: a multiinstitutional analysis by the Japan p53 Antibody Research Group. Cancer 97(3):682–689
Soussi T (2000) p53 antibodies in the sera of patients with various types of cancer: a review. Cancer Res 60(7):1777–1788
Yamaoka K, Mitsunobu F, Kojima S, Shibakura M, Kataoka T, Hanamoto K et al (2005) The elevation of p53 protein level and SOD activity in the resident blood of the Misasa radon hot spring district. J Radiat Res (Tokyo) 46(1):21–24
Li Y, Karjalainen A, Koskinen H, Hemminki K, Vainio H, Shnaidman M et al (2005) p53 autoantibodies predict subsequent development of cancer. Int J Cancer 114(1):157–160
El Far MA, Atwa MA, Yahya RS, El Basuni MA (2006) Evaluation of serum levels of p53 in hepatocellular carcinoma in Egypt. Clin Chem Lab Med 44(5):653–656
Sakamoto M (2001) P53 in sera for the early detection of hepatocellular carcinoma. J Gastroenterol 36(12):865–866
Goodell V, Salazar LG, Urban N, Drescher CW, Gray H, Swensen RE et al (2006) Antibody immunity to the p53 oncogenic protein is a prognostic indicator in ovarian cancer. J Clin Oncol 24(5):762–768
Chae BJ, Bae JS, Lee A, Park WC, Seo YJ, Song BJ et al (2009) p53 as a specific prognostic factor in triple-negative breast cancer. Jpn J Clin Oncol 39(4):217–224
Shen YC, Hu FC, Jeng YM, Chang YT, Lin ZZ, Chang MC et al (2009) Nuclear overexpression of mitotic regulatory proteins in biliary tract cancer: correlation with clinicopathologic features and patient survival. Cancer Epidemiol Biomarkers Prev 18(2):417–423
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This study was supported by the Bureau of Science and Technology of Guangzhou City (Grant No. 2000-Z-111-01).
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Wu, M., Mao, C., Chen, Q. et al. Serum p53 protein and anti-p53 antibodies are associated with increased cancer risk: a case–control study of 569 patients and 879 healthy controls. Mol Biol Rep 37, 339–343 (2010). https://doi.org/10.1007/s11033-009-9744-7
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DOI: https://doi.org/10.1007/s11033-009-9744-7